Evaluation of radiation-induced chromosomal aberrations in human peripheral blood lymphocytes in vitro results of an IAEA-coordinated programme
| Autor: | R.N. Mukherjee, Adayapalam T. Natarajan, Günter Obe, P.C. Gooch, J Pohl-Rüling, P.P.W. van Buul, Sayaka Nakai, H G Schwarzacher, Ei-Ichi Takahashi, Albert Léonard, F. Palitti, Caterina Tanzarella, T. Sharma, Néstor O. Bianchi, M Bianchi, U. Mukherjee, M. Kučerová, J. G. Brewen, L. Fabry, P. Fischer, K.E. Buckton, David R. Scott |
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| Rok vydání: | 1982 |
| Předmět: |
International Cooperation
Health Toxicology and Mutagenesis Aberrant cell Argentina Radiation induced Fixation time In Vitro Techniques Biology Chromosomes Andrology Japan Reference Values Genetics Humans Lymphocytes Molecular Biology Mitosis Absorbed Radiation Dose Cells Cultured Fixation (histology) Chromosome Aberrations X-Rays Dose-Response Relationship Radiation United Kingdom United States Peripheral blood In vitro Austria Immunology |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 96:233-242 |
| ISSN: | 0027-5107 |
| Popis: | The results of an IAEA coordinated programme on radiation induced chromosomal aberrations in human peripheral blood lymphocytes in vitro are presented. In a master experiment, a whole blood sample from one donor was irradiated with 200 R of X-rays. Different fixation times from 46 to 82 h were used. The progression of cells into mitosis was monitored by BrdUrd incorporation. 14 investigators took part in the scoring of chromosomal aberrations. The main conclusions of this study are: (1) The mean frequencies of aberrations changed with fixation time. (2) The number of cells scored as aberrant by different laboratories was very similar, but there was variability in the number of aberrations scored per aberrant cell. (3) The differences in the frequencies of aberrations between laboratories were minimal when the scoring was restricted to the first major peak of mitotic activity and sufficient cells were scored. It is concluded that using controlled experimental conditions, human peripheral blood lymphocytes can effectively be used as a reliable biological dosimeter for absorbed radiation dose. |
| Databáze: | OpenAIRE |
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