Tri-layered chitosan scaffold as a potential skin substitute
| Autor: | Hsin-Yi Lin, Shih-Hsin Chang, Sheng-Tung Huang, Shin-Hung Chen |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Scaffold
Materials science Composite number Biomedical Engineering Biophysics Nanofibers Bioengineering macromolecular substances Cell Line Biomaterials Chitosan chemistry.chemical_compound Elastic Modulus Tensile Strength Ultimate tensile strength Materials Testing medicine Composite material Acetic Acid Cell Proliferation Skin Artificial Tissue Scaffolds technology industry and agriculture Water DNA Fibroblasts equipment and supplies Electrospinning carbohydrates (lipids) medicine.anatomical_structure Spectrometry Fluorescence chemistry Nanofiber Microscopy Electron Scanning Pectins Epidermis Stress Mechanical Layer (electronics) Porosity |
| Zdroj: | Journal of biomaterials science. Polymer edition. 26(13) |
| ISSN: | 1568-5624 |
| Popis: | A tri-layered chitosan-based scaffold was successfully made to replicate the striation of a full-thickness skin more accurately than a single- or bi-layered scaffold, which needed weeks of co-culturing of fibroblasts and keratinocytes to achieve similar striation. Chitosan solution was freeze-dried and made into porous disks. Chitosan or chitosan-pectin in acetic acid solution was electrospun onto the chitosan disk to form a nanofibrous layer and a thin film. Examinations based on scanning electron spectroscopy showed that the scaffold was composed of a porous layer (2 mm) to simulate the dermis, a thin film (25-45 μm) to mimic the basement membrane, and a layer of nanofibers (100-200 μm) to serve as the protective epidermis. The tensile strength and modulus of the composite scaffold were significantly higher than those of the chitosan disk (p 0.01). The composite was able to quickly absorb water and stayed intact throughout the course of the 14-day cell culture tests. The fibroblast cells seeded on both sides of the scaffolds were able to proliferate and stayed separated by the thin film. |
| Databáze: | OpenAIRE |
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