Increased Expression of thehPim-2Gene In Human Chronic lymphocytic Leukemia and Non-Hodgkin Lymphoma
| Autor: | Sarah Shalom, Ariel Schwartz, Alona Kremer, Borislava Grinblat, Hanna Bessler, Donald A. Kristt, Jeremy Don, Amos Cohen, Drorit Merkel, Marisa Halperin |
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| Rok vydání: | 2004 |
| Předmět: |
Cancer Research
Chronic lymphocytic leukemia Protein Serine-Threonine Kinases Biology immune system diseases Proto-Oncogene Proteins hemic and lymphatic diseases Gene expression medicine Humans RNA Neoplasm Gene In Situ Hybridization Reverse Transcriptase Polymerase Chain Reaction Lymphoma Non-Hodgkin Hematology medicine.disease Leukemia Lymphocytic Chronic B-Cell Up-Regulation Lymphoma Non-Hodgkin's lymphoma Gene Expression Regulation Neoplastic Leukemia Oncology Immunology Hodgkin lymphoma Lymph Nodes |
| Zdroj: | Leukemia & Lymphoma. 45:951-955 |
| ISSN: | 1029-2403 1042-8194 |
| DOI: | 10.1080/10428190310001641251 |
| Popis: | Pim-1 and Pim-2 are murine proto-oncogenes implicated in lymphomagenesis. The aim of this study was to investigate whether the human Pim-2 (hPim-2) expression is altered in chronic lymphocytic leukemia (B-CLL) and non-Hodgkin's lymphomas (NHL). We analyzed hPim-2 expression in 48 patients with NHL and CLL by quantitative in-situ hybridization, quantitative RT-PCR and FACS analysis. In-situ hybridization revealed a 5.5 +/- 2.2 times higher expression of hPim-2 in NHL over normal lymphocytes (P0.001). Similarly, with quantitative RT-PCR, expression in NHL was 1.5 to 2.6 times higher in involved splenic foci compared to nearby uninvolved regions (n = 3). hPim-2 mRNA was increased 3-folds in B-CLL over normal B-cells (P0.006). The increased hPim-2 levels correlated with lymphocyte doubling time (DT), in that mRNA levels were two times greater in patients with rapid DT (P0.006). Moreover, a significant correlation was found between hPim-2 expression and the Binet staging system of CLL (P0.022). The hPIM-2-protein expression was also upregulated in CLL, as assessed by FACS analysis. Therefore, this report provides direct evidence for a linkage of hPim-2 upregulation to NHL and CLL in man. This relationship between hPim-2 and NHL and CLL raises a number of novel mechanistic options for the genesis and/or progression of some types of human lymphomas. |
| Databáze: | OpenAIRE |
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