Biomarkers of neurotoxic shellfish poisoning
| Autor: | Leanne J. Flewelling, Kevin D. White, Steven M. Plakas, Edward L.E. Jester, Hudson R. Granade, Ann Abraham, Kathleen R. El Said, Robert Wayne Dickey |
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| Rok vydání: | 2008 |
| Předmět: |
Urinary system
Neurotoxic shellfish poisoning Neurotoxins Enzyme-Linked Immunosorbent Assay Urine Biology Toxicology medicine.disease_cause complex mixtures Foodborne Diseases Brevetoxin medicine Animals Shellfish Poisoning Shellfish Chromatography Molecular Structure Toxin Oxocins Bivalvia Toxicity Dinoflagellida Biomarker (medicine) Marine Toxins Biomarkers |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 52(2) |
| ISSN: | 0041-0101 |
| Popis: | Urine specimens from patients diagnosed with neurotoxic shellfish poisoning (NSP) were examined for biomarkers of brevetoxin intoxication. Brevetoxins were concentrated from urine by using solid-phase extraction (SPE), and analyzed by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Urine extracts were fractionated by LC, and fractions analyzed for brevetoxins by ELISA. In subsequent LC-MS/MS analyses, several brevetoxin metabolites of B-type backbone were identified, with elution profiles consistent with those of ELISA. The more abundant brevetoxin metabolites in urine were characterized structurally by LC-MS/MS. With the exception of BTX-3, brevetoxin metabolites in urine differed from those found in shellfish and in shellfish meal remnants. Proposed structures of these major urinary metabolites are methylsulfoxy BTX-3, 27-epoxy BTX-3, and reduced BTX-B5. BTX-3 was found in all specimens examined. BTX-3 concentrations in urine, as determined by LC-MS/MS, correlated well with composite toxin measurements by ELISA ( r 2 = 0.96). BTX-3 is a useful biomarker for confirmation of clinical diagnosis of NSP. |
| Databáze: | OpenAIRE |
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