Diagnostic Performances of Hepatitis C Virus-NS4 Antigen in Patients with Different Liver Pathologies
| Autor: | Mohga S Abdalla, El-Sayed M. Moussa, Mohamed M. Omran, Ibrahim El-Dosoky, Mohamed F. Ghaly, Wesam A. Nasif, Wageh El-Shony, Eman Nour, Ehab B. El-Domany, Hayat M. Sharada, Abd El-Raheem R. El-Shanshoury, Khaled Farid, Abdelfattah M. Attallah |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Liver Cirrhosis Male Pathology medicine.medical_specialty Carcinoma Hepatocellular Cirrhosis Hepatitis C virus Blotting Western Enzyme-Linked Immunosorbent Assay Viremia Hepacivirus Viral Nonstructural Proteins medicine.disease_cause Young Adult Liver disease Antigen Humans Medicine business.industry Liver Diseases Liver Neoplasms virus diseases General Medicine Hepatitis C Gold standard (test) Hepatitis C Chronic Middle Aged medicine.disease digestive system diseases Hepatocellular carcinoma Egypt Female Hepatitis C Antigens business |
| Zdroj: | Archives of Medical Research. 43:555-562 |
| ISSN: | 0188-4409 |
| Popis: | Background and Aims Hepatitis C virus (HCV) has emerged as the major pathogen of liver disease worldwide. The aim of this study was to quantitate and evaluate the performance of HCV-NS4 antigen as an alternative approach for confirmation of viremia. Methods Detection of HCV-NS4 was assessed in 883 patients with chronic hepatitis C. Areas under the ROC curves (AUC) were used to assess and compare diagnostic accuracy of ELISA for HCV-NS4 with quantitative HCV-RNA as a gold standard. Results HCV-NS4 was identified at 27 kDa using Western blot. AUC for HCV-NS4 detection was 0.95 for all patients with different liver pathologies: 0.93 for liver fibrosis (LF), 0.95 for liver cirrhosis (LC) and 0.98 for hepatocellular carcinoma (HCC). The mean ± SD (μg/mL) of HCV-NS4 in LF was 94.2 ± 55.6; in LC was 99.3 ± 64.8 and in HCC was 124.9 ± 70.3. Conclusions HCV-NS4 antigen detection using ELISA is a reliable test in the confirmation of HCV infection. |
| Databáze: | OpenAIRE |
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