Photoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs
| Autor: | Yasushi Sako, Peter Greimel, Toshihide Kobayashi, Takehiko Inaba, Fumihiro Fujimori, Asami Makino, Mariko Sumi |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Multidisciplinary
Quenching (fluorescence) 010405 organic chemistry Chemistry Science Phospholipid 010402 general chemistry 01 natural sciences Photobleaching Fluorescence Article 0104 chemical sciences chemistry.chemical_compound Photochromism Förster resonance energy transfer Biochemistry Membrane region Biophysics Medicine lipids (amino acids peptides and proteins) Biophysical chemistry |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | We have developed and characterized a novel photoswitchable phospholipid analog termed N-nitroBIPS-DPPG. The fluorescence can be switched on and off repeatedly with minimal photobleaching by UV or visible light exposure, respectively. The rather large photochromic head group is inserted deeply into the interfacial membrane region conferring a conical overall lipid shape, preference for a positive curvature and only minimal intermembrane transfer. Utilizing the switchable NBD fluorescence quenching ability of N-nitroBIPS-DPPG, a detergent free intermembrane transfer assay system for NBD modified lipids was demonstrated and validated. As NBD quenching can be turned off, total NBD associated sample fluorescence can be determined without the need of detergents. This not only reduces detergent associated systematic errors, but also simplifies assay handling and allows assay extension to detergent insoluble lipid species. |
| Databáze: | OpenAIRE |
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