A Plausible Antibacterial Green Synthesized AgNPs from Tridax procumbens Leaf-flower Extract
| Autor: | Jesvin Shobini, Jesse Joel T |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
010304 chemical physics biology Traditional medicine Chemistry green synthesis 030111 toxicology Tridax procumbens tridax procumbens biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Microbiology QR1-502 antibacterial 03 medical and health sciences 0103 physical sciences leaf flower Biotechnology |
| Zdroj: | Journal of Pure and Applied Microbiology, Vol 12, Iss 4, Pp 2135-2142 (2018) |
| ISSN: | 2581-690X 0973-7510 |
| DOI: | 10.22207/jpam.12.4.51 |
| Popis: | The current study was aimed at the green synthesis of silver nanoparticles (AgNPs) from leaf-flower and flower extracts of Tridax procumbens which are effective against oral pathogens and its characterization. The silver nanoparticle attaches to the cell membrane of bacteria and disturbs its function by penetrating inside bacterial cell by releasing silver ions and inhibits the bacterial growth. The silver nanoparticles obtained from the bio-reduction method using leaf-flower and flower extract with silver nitrate was characterized using UV- visible spectroscopy (UV-Vis), X-Ray Diffraction (XRD), Fourier Transform Infra-Red spectroscopy (FTIR), Scanning Electron Microscope (SEM). The presence of AgNPs in the aqueous extract was confirmed by the yellowish-brown color change of the extract and by Surface Plasmon Resonance (SPR) peaks in the UV- Visible spectroscopy (430nm-510nm) analysis, X-Ray Diffraction (XRD) for Functional groups, Fourier Transform Infra-Red spectroscopy (FTIR) for Phytophenols (3448.72/cm). Scanning Electron Microscope (SEM) showed particle range 33-45nm and particle size analysis using Zeta potential (142.2 (d.nm). The other analysis showed that the AgNPs were irregularly shaped, crystalline in nature and less stabilized due the effect of capping agents in the extracts. In this study, the antibacterial activity of these nanoparticles was tested against the oral pathogens. The antibiotic resistance in pathogenic microbes has led to the emergence of multidrug-resistant (MDR) pathogens. Therefore, the Leaf-flower combination was seen as plausible antibacterial agent in the prevention of Dental caries and can be used as an additive in Tooth paste or mouth freshners. |
| Databáze: | OpenAIRE |
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