Wedelolactone inhibits adipogenesis through the ERK pathway in human adipose tissue‐derived mesenchymal stem cells
| Autor: | Yun-Hee Kim, Seyoung Lim, Kyungmoo Yea, Hyun-Jun Jang, Eung-Kyun Kim, Whaseon Lee-Kwon, Jung-Min Kim, Eun Hee Park, Jung Kuk Kim, Sung Ho Ryu, Pann-Ghill Suh |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
MAPK/ERK pathway medicine.medical_specialty MAP Kinase Signaling System Cellular differentiation Primary Cell Culture Subcutaneous Fat Adipose tissue Peroxisome proliferator-activated receptor Biology Fatty Acid-Binding Proteins Biochemistry chemistry.chemical_compound Coumarins Adipocyte Internal medicine Adipocytes CCAAT-Enhancer-Binding Protein-alpha medicine Humans Molecular Biology Flavonoids Mitogen-Activated Protein Kinase 1 chemistry.chemical_classification Adipogenesis Mitogen-Activated Protein Kinase 3 Cell Differentiation Mesenchymal Stem Cells Cell Biology Middle Aged Wedelolactone Cell biology PPAR gamma Lipoprotein Lipase Endocrinology Gene Expression Regulation chemistry Cancer cell Female Anti-Obesity Agents |
| Zdroj: | Journal of Cellular Biochemistry. 113:3436-3445 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.24220 |
| Popis: | Wedelolactone is an herbal medicine that is used to treat septic shock, hepatitis and venom poisoning. Although in differentiated and cancer cells, wedelolactone has been identified as anti-inflammatory, growth inhibitory, and pro-apoptotic, the effects of wedelolactone on stem cell differentiation remain largely unknown. Here, we report that wedelolactone inhibits the adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAMSCs). Wedelolactone reduced the formation of lipid droplets and the expression of adipogenesis-related proteins, such as CCAAT enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), lipoprotein lipase (LPL), and adipocyte fatty acid-binding protein aP2 (aP2). Wedelolactone mediated this process by sustaining ERK activity. In addition, inhibition of ERK activity with PD98059 resulted in reversion of the wedelolactone-mediated inhibition of adipogenic differentiation. Taken together, these results indicate that wedelolactone inhibits adipogenic differentiation through ERK pathway and suggest a novel inhibitory effect of wedelolactone on adipogenic differentiation in hAMSCs. |
| Databáze: | OpenAIRE |
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