Comparative analytical evaluation of the respiratory TaqMan Array Card with real-time PCR and commercial multi-pathogen assays
| Autor: | Jeffrey L. Benfer, Remedios B. Gose, Rebecca Sciulli, Stephanie Chester, Marisela Ansbro, Kathleen Tatti, Jonas M. Winchell, Erik Reisdorf, Xin Wang, Lucy E. DesJardin, Kimberly Doan, John J. Harvey, Michael Popowich, Daniel Jernigan, Leonard W. Mayer, Kirsten St. George, Velusamy Srinivasan, Ailyn C. Pérez-Osorio, Tricia Aden, Joshua Hall, Stephen A. Burke, Zhen Li, Jing Bai, Alison C. Mawle, Tammy Quinlan, William A. Glover, Sandra Smole, Tanya A. Halse, Maria Lucia Tondella, Cynthia Hatcher, Brett Whitaker, Thomas Whyte, Denny Russell |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Respiratory pathogens 030106 microbiology Microfluidics Computational biology Biology Analytical techniques Real-Time Polymerase Chain Reaction Sensitivity and Specificity Article 03 medical and health sciences Virology TaqMan Humans Pathogen Respiratory Tract Infections Oligonucleotide Array Sequence Analysis Bacteria Reproducibility of Results Disease control United States 3. Good health Comparative studies 030104 developmental biology Real-time polymerase chain reaction PCR Immunology Viruses Centers for Disease Control and Prevention U.S |
| Zdroj: | Journal of Virological Methods |
| ISSN: | 1879-0984 |
| Popis: | Highlights • Viral and bacterial real-time PCR oligonucleotides were spotted on TaqMan Array Cards. • Analytical sensitivity was compared with standalone laboratory PCR assays. • TaqMan Array Card sensitivity was generally one log lower. • Reproducibility across six independent testing sites was within one log. In this study, a multicenter evaluation of the Life Technologies TaqMan® Array Card (TAC) with 21 custom viral and bacterial respiratory assays was performed on the Applied Biosystems ViiA™ 7 Real-Time PCR System. The goal of the study was to demonstrate the analytical performance of this platform when compared to identical individual pathogen specific laboratory developed tests (LDTs) designed at the Centers for Disease Control and Prevention (CDC), equivalent LDTs provided by state public health laboratories, or to three different commercial multi-respiratory panels. CDC and Association of Public Health Laboratories (APHL) LDTs had similar analytical sensitivities for viral pathogens, while several of the bacterial pathogen APHL LDTs demonstrated sensitivities one log higher than the corresponding CDC LDT. When compared to CDC LDTs, TAC assays were generally one to two logs less sensitive depending on the site performing the analysis. Finally, TAC assays were generally more sensitive than their counterparts in three different commercial multi-respiratory panels. TAC technology allows users to spot customized assays and design TAC layout, simplify assay setup, conserve specimen, dramatically reduce contamination potential, and as demonstrated in this study, analyze multiple samples in parallel with good reproducibility between instruments and operators. |
| Databáze: | OpenAIRE |
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