AtPER1 enhances primary seed dormancy and reduces seed germination by suppressing the ABA catabolism and GA biosynthesis in Arabidopsis seeds
| Autor: | Huhui Chen, Jun Liu, Pu Chu, Zhenwei Liang, Shangzhi Huang, Jianhua Tong, Langtao Xiao, Chenlong Li, Wei Fu, Jiuxiao Ruan, Yin Li |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Arabidopsis Germination Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Cytochrome P-450 Enzyme System Plant Growth Regulators Gene Expression Regulation Plant Genetics Arabidopsis thaliana Abscisic acid Plant Proteins Arabidopsis Proteins Catabolism fungi Seed dormancy food and beverages Cell Biology Plant Dormancy biology.organism_classification Gibberellins Horticulture Phenotype 030104 developmental biology chemistry Seedlings Mutation Seeds Gibberellin Reactive Oxygen Species Transcriptome Abscisic Acid 010606 plant biology & botany |
| Zdroj: | The Plant Journal. 101:310-323 |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1111/tpj.14542 |
| Popis: | Seed is vital to the conservation of germplasm and plant biodiversity. Seed dormancy is an adaptive trait in numerous seed-plant species, enabling plants to survive under stressful conditions. Seed dormancy is mainly controlled by abscisic acid (ABA) and gibberellin (GA) and can be classified as primary and secondary seed dormancy. The primary seed dormancy is induced by maternal ABA. Here we found that AtPER1, a seed-specific peroxiredoxin, is involved in enhancing primary seed dormancy. Two loss-of-function atper1 mutants, atper1-1 and atper1-2, displayed suppressed primary seed dormancy accompanied with reduced ABA and increased GA contents in seeds. Furthermore, atper1 mutant seeds were insensitive to abiotic stresses during seed germination. The expression of several ABA catabolism genes (CYP707A1, CYP707A2, and CYP707A3) and GA biosynthesis genes (GA20ox1, GA20ox3, and KAO3) in atper1 mutant seeds was increased compared to wild-type seeds. The suppressed primary seed dormancy of atper1-1 was completely reduced by deletion of CYP707A genes. Furthermore, loss-of-function of AtPER1 cannot enhance the seed germination ratio of aba2-1 or ga1-t, suggesting that AtPER1-enhanced primary seed dormancy is dependent on ABA and GA. Additionally, the level of reactive oxygen species (ROS) in atper1 mutant seeds was significantly higher than that in wild-type seeds. Taken together, our results demonstrate that AtPER1 eliminates ROS to suppress ABA catabolism and GA biosynthesis, and thus improves the primary seed dormancy and make the seeds less sensitive to adverse environmental conditions. |
| Databáze: | OpenAIRE |
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