Cellular Mechanisms of Aortic Valve Calcification

Autor: Olga Moiseeva, E Zhiduleva, M. L. Gordeev, E. V. Ignat’eva, Aleksandra Kostina, Alexey Golovkin, Anna Kostareva, Anna Malashicheva, A. Shishkova, Olga Irtyuga, K. A. Levchuk, A. Yu. Rylov
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Aortic valve
Pathology
medicine.medical_specialty
medicine.medical_treatment
Primary Cell Culture
Bone Morphogenetic Protein 2
Core Binding Factor Alpha 1 Subunit
Ascorbic Acid
Bone morphogenetic protein 2
Dexamethasone
General Biochemistry
Genetics and Molecular Biology
Pathogenesis
03 medical and health sciences
Osteoprotegerin
Osteogenesis
Humans
Medicine
Aged
Heart transplantation
Osteoblasts
business.industry
Calcinosis
Membrane Proteins
Cell Differentiation
Aortic Valve Stenosis
General Medicine
Middle Aged
Phosphoproteins
medicine.disease
RUNX2
Stenosis
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
Aortic Valve
Glycerophosphates
cardiovascular system
Heart Transplantation
Female
Osteopontin
Tricuspid Valve
Stromal Cells
Aortic valve calcification
business
Zdroj: Bulletin of Experimental Biology and Medicine. 164:371-375
ISSN: 1573-8221
0007-4888
Popis: Comparative in vitro study examined the osteogenic potential of interstitial cells of aortic valve obtained from the patients with aortic stenosis and from control recipients of orthotopic heart transplantation with intact aortic valve. The osteogenic inductors augmented mineralization of aortic valve interstitial cells (AVIC) in patients with aortic stenosis in comparison with the control level. Native AVIC culture of aortic stenosis patients demonstrated overexpression of osteopontin gene (OPN) and underexpression of osteoprotegerin gene (OPG) in comparison with control levels. In both groups, AVIC differentiation was associated with overexpression of RUNX2 and SPRY1 genes. In AVIC of aortic stenosis patients, expression of BMP2 gene was significantly greater than the control level. The study revealed an enhanced sensitivity of AVIC to osteogenic inductors in aortic stenosis patients, which indicates probable implication of OPN, OPG, and BMP2 genes in pathogenesis of aortic valve calcification.
Databáze: OpenAIRE
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