Retinoic acid signalling is a candidate regulator of the expression of pituitary-specific transcription factor Prop1 in the developing rodent pituitary
| Autor: | Yukio Kato, Takashi Yashiro, Ken Fujiwara, Hiroto Nishihara, Takako Kato, Saishu Yoshida |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Receptors Retinoic Acid Endocrinology Diabetes and Metabolism Retinoic acid Tretinoin Biology Mice 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Anterior pituitary Transcription (biology) Internal medicine Gene expression Transcriptional regulation medicine Animals Rats Wistar Receptor Transcription factor Homeodomain Proteins Reporter gene Endocrine and Autonomic Systems Gene Expression Regulation Developmental Rats Cell biology Retinoid X Receptors 030104 developmental biology medicine.anatomical_structure chemistry Pituitary Gland Signal Transduction |
| Zdroj: | Journal of Neuroendocrinology. 30:e12570 |
| ISSN: | 0953-8194 |
| DOI: | 10.1111/jne.12570 |
| Popis: | Development of the anterior pituitary proceeds via spatiotemporal patterning of transcription factors and signalling molecules. Among them, retinoic acid (RA) functions as an important signalling molecule for vertebrate organogenesis in many tissues. However, little is known regarding the target genes in the developing pituitary. The present study aimed to clarify the relationship between endogenous RA signalling and mRNA expression of the pituitary-specific transcription factor Prop1 in the pituitary primordium of Rathke's pouch. Gene expression analysis and in situ hybridisation demonstrated that retinaldehyde dehydrogenases (Raldhs) and all types of RA receptors (Rars) are expressed at the level of transcription in the rat Rathke's pouch. Ex vivo organ culture using Rathke's pouch and an in vitro reporter assay demonstrated that RA signalling increases the expression level of Prop1 via RARα. Moreover, a reporter assay using serial truncated constructs of the 5'-upstream region of mouse Prop1 revealed a predicted cis-regulatory element of RARα. This is the first report of a relationship between RA signalling and Prop1-expression during early pituitary development. |
| Databáze: | OpenAIRE |
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