A mouse model of vitamin D insufficiency: is there a relationship between 25(OH) vitamin D levels and obesity?
| Autor: | Ping Yu, Kenneth L. Seldeen, Maria Rodríguez-Gonzalez, Zachary Sheridan, Mireya Hernandez, Bruce R. Troen, Manhui Pang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Vitamin medicine.medical_specialty Calorie Bone density Calcitriol Endocrinology Diabetes and Metabolism Medicine (miscellaneous) 030209 endocrinology & metabolism BMI Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Vitamin D and neurology Obesity Vitamin D Nutrition and Dietetics business.industry Research High fat diet Weight Insufficiency 030104 developmental biology Endocrinology chemistry Fat Body Composition Lean body mass business Cholecalciferol Body mass index PTH medicine.drug |
| Zdroj: | Nutrition & Metabolism |
| ISSN: | 1743-7075 |
| Popis: | Background Vitamin D insufficiency (serum 25-OH vitamin D > 10 ng/ml and 30 kg/m2), yet relationships between the two are poorly understood. Objectives of this study include identification of the impact of obesity on reducing serum 25-OH vitamin D concentration, particularly in response to altered vitamin D3 supplementation, and to elucidate the longitudinal impact of serum 25-OH vitamin D on body mass index. Methods Twenty four-week-old lean and obese male C57BL/6 J mice were fed low, standard, or high levels of cholecalciferol supplementation and followed for 24 weeks. Longitudinal measurements include serum 25-OH and 1,25-(OH)2 vitamin D, intact PTH, and calcium concentrations, as well as BMI, bone density and body fat/lean mass. Results Baseline serum 25-OH concentrations were not different in lean and obese mice (lean 32.8 ± 4.4 ng/ml versus obese 30.9 ± 1.6 ng/ml p = 0.09). Lean mice receiving low supplementation exhibited rapid declines in serum 25-OH vitamin D concentrations, falling from 33.4 ± 5.4 ng/ml to 14.5 ± 3.4 ng/ml after 2 weeks, while obese mice declined at a lower rate, falling from 30.9 ± 1.5 to 19.0 ± 0.9 ng/ml within the same time period. Surprisingly, high vitamin D3 supplementation did not substantially increase serum vitamin D concentrations above standard supplementation, in either lean or obese mice. No differences in serum 1,25-(OH)2 vitamin D, intact parathyroid hormone (PTH) or serum calcium were observed between lean and obese mice within the same vitamin D supplementation group. Yet obese mice exhibited lower serum calcitriol, higher serum PTH, and lower bone mineral density (BMD) than did lean mice. Additionally, neither body mass index nor body fat % was significantly correlated with vitamin D concentrations. Interestingly, lean mice with high vitamin D supplementation consumed significantly more food than did lean mice with standard or low supplementation (14.6 ± 1.7 kcal/mouse/day versus 11.8 ± 1.4 and 12.3 ± 1.7 respectively, p |
| Databáze: | OpenAIRE |
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