Puerarin, isolated from Pueraria lobata (Willd.), protects against diabetic nephropathy by attenuating oxidative stress
| Autor: | Xiaohui Xu, Hai Kuang, Zhaoni Chen, Ni Zheng, Wansu Huang, Tao Liang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine medicine.medical_specialty Renal cortex Biology Protective Agents medicine.disease_cause Blood Urea Nitrogen Diabetes Mellitus Experimental Diabetic nephropathy Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Puerarin Internal medicine Genetics medicine Animals Diabetic Nephropathies Blood urea nitrogen chemistry.chemical_classification Kidney Reactive oxygen species Molecular Structure General Medicine medicine.disease Streptozotocin Isoflavones Mice Inbred C57BL Oxidative Stress Pueraria 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Creatinine 030220 oncology & carcinogenesis Oxidative stress medicine.drug |
| Zdroj: | Gene. 591:411-416 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2016.06.032 |
| Popis: | In this study, we evaluated the effect of puerarin (PR) on diabetic nephropathy (DN) in streptozotocin (STZ)-induced diabetic mice. The fasting blood glucose (FBG), blood urea nitrogen (BUN) and serum creatinine (Scr), as well as 24-hour urine protein levels were effectively ameliorated in DN mice treated with PR (20, 40, 80mg/kg/day). Furthermore, PR treatment markedly resulted in down-regulation of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and reactive oxygen species (ROS) in kidney. Interestingly, the activities of manganese superoxide dismutase (MnSOD) and catalase (CAT) were increased by PR. An improvement in kidney tissue damage could be observed after PR administration. Further ultrastructural investigation revealed a dramatically ameliorative effect of PR on mitochondrial damage. Meanwhile, the silent information regulator 1 (SIRT1), forkhead box protein O1 (FOXO1) and alpha subunit of peroxisome proliferators-activated receptor-gamma coactivator-1 (PGC-1α) expressions were significantly up-regulated at protein level by PR administration in renal cortex. However, the protein expression of nuclear-factor kappa B (NF-κB) was down-regulated in PR groups. Our present study demonstrates the hypoglycemic and renal protective effects of PR in DN mice, which support its anti-diabetic property. PR exerts its renal protection effect probably via the mechanism of attenuating SIRT1/FOXO1 pathway for renal protection. |
| Databáze: | OpenAIRE |
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