Multimodal Label-Free Imaging for Detecting Maturation of Engineered Osteogenic Grafts
| Autor: | Marissa Gionet-Gonzales, J. Kent Leach, Jenna N. Harvestine, Kyriacos A. Athanasiou, Cai Li, Jerry C. Hu, Clay S Sheaff, Anne K. Haudenschild, Laura Marcu |
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| Rok vydání: | 2019 |
| Předmět: |
mesenchymal stem/stromal cell
Fluorescence-lifetime imaging microscopy Stromal cell 0206 medical engineering Biomedical Engineering osteogenic differentiation Bioengineering 02 engineering and technology Matrix (biology) Regenerative Medicine Article Biomaterials Microscopy ultrasound backscatter osteogenic graft Label free Multimodal imaging Chemistry Mesenchymal stem cell 021001 nanoscience & nanotechnology Stem Cell Research 020601 biomedical engineering fluorescence lifetime imaging 4.1 Discovery and preclinical testing of markers and technologies Musculoskeletal Ultrasound backscatter Biomedical Imaging 0210 nano-technology Biomedical engineering |
| Zdroj: | ACS biomaterials science & engineering, vol 5, iss 4 ACS Biomater Sci Eng |
| Popis: | There is a critical need to develop noninvasive, nondestructive methods for assessing the quality of engineered constructs prior to implantation. Currently, the composition and maturity of engineered tissues are assessed using destructive, costly, and time-consuming biochemical and mechanical analyses. The goal of this study was to use noninvasive, multimodal imaging to monitor osteogenic differentiation and matrix deposition by human mesenchymal stem/stromal cells (MSCs) during in vitro culture. MSCs were encapsulated in alginate hydrogels and cultured in osteogenic conditions for 4 weeks. Samples were evaluated using fluorescence lifetime imaging (FLIm) and ultrasound backscatter microscopy (UBM) prior to traditional biochemical and mechanical testing. Using linear regression analysis, we identified strong correlations between imaging parameters (e.g., fluorescence lifetime and acoustic attenuation coefficient) and destructive mechanical and biochemical tests to assess the maturation of osteogenically induced constructs. These data demonstrate the promise of nondestructive label-free imaging techniques to noninvasively ascertain the progression and maturity of tissue engineered bone grafts. |
| Databáze: | OpenAIRE |
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