Loliolide Presents Antiapoptosis and Antiscratching Effects in Human Keratinocytes
| Autor: | Jong-Hoon Kim, Laura Rojas Lorz, Kyung Ja Park, Mohammad Amjad Hossain, Eunju Choi, Young Im Choi, SeokGu Jang, Jae Youl Cho, Hye Yeon Lim, Keejung Yoon, Dong Sam Kim, Sang Hee Park, Sunggyu Kim |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Keratinocytes
Cell Survival Gene Expression antioxidant activity Apoptosis macromolecular substances Article Antioxidants Catalysis Cell Line Inorganic Chemistry lcsh:Chemistry antiscratching effect chemistry.chemical_compound antiapoptosis Cell Movement Humans Physical and Theoretical Chemistry Molecular Biology Protein kinase B lcsh:QH301-705.5 antiwrinkling activity Spectroscopy PI3K/AKT/mTOR pathway Benzofurans chemistry.chemical_classification Reactive oxygen species Molecular Structure integumentary system Organic Chemistry General Medicine Matrix Metalloproteinases Computer Science Applications Cell biology Blot HaCaT chemistry lcsh:Biology (General) lcsh:QD1-999 Keratinocyte growth factor Signal transduction |
| Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 3 International Journal of Molecular Sciences, Vol 20, Iss 3, p 651 (2019) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms20030651 |
| Popis: | Loliolide is a monoterpenoid hydroxylactone present in freshwater algae that has anti-inflammatory and antiaging activity however, its effects on ultraviolet-damaged skin have yet to be elucidated. This study investigated the antiapoptosis and wound-healing effects of loliolide using HaCaT cells (a human keratinocyte cell line). Loliolide inhibited the expression of reactive oxygen species (ROS) induced by ultraviolet radiation as well as wrinkle formation-related matrix metalloproteinase genes and increased the expression of the damage repair-related gene SIRT1. The apoptosis signaling pathway was confirmed by Western blot analysis, which showed that loliolide was able to reduce the expression of caspases 3, 8, and 9, which are related to ROS-induced apoptosis. In addition, Western blotting, reverse-transcription polymerase chain reaction (PCR), and real-time PCR analyses showed that loliolide enhanced the expression of the epidermal growth factor receptor signaling pathway (PI3K, AKT) and migration factors, such as K6, K16, and K17 keratinocyte growth factor and inflammatory cytokines, such as interleukin (IL)-1, IL-17, and IL-22 expressed during the cellular scratching process, suggesting a putative wound-healing ability. Because of the antiapoptosis and antiscratching effects on skin of both loliolide and loliolide-rich Prasiola japonica ethanol extract, we consider the former to be an important compound used in the cosmeceutical industry. |
| Databáze: | OpenAIRE |
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