Optimization of Pancreatic Juice Collection: A First Step Toward Biomarker Discovery and Early Detection of Pancreatic Cancer
| Autor: | Maikel P. Peppelenbosch, Iris J. Levink, Maurice P.H.M. Jansen, Jan-Werner Poley, Lydi M.J.W. van Driel, Celio J C Fernandes, Marco J. Bruno, Anieta M. Sieuwerts, Kateryna Nesteruk, Gwenny M. Fuhler, Dido I Visser, Djuna L. Cahen |
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| Přispěvatelé: | Gastroenterology & Hepatology, Medical Oncology |
| Rok vydání: | 2020 |
| Předmět: |
Endoscopic ultrasound
Suction (medicine) medicine.medical_specialty Endoscope Endosonography 03 medical and health sciences 0302 clinical medicine SDG 3 - Good Health and Well-being Pancreatic Juice Pancreatic cancer medicine Humans Prospective Studies Stage (cooking) Biomarker discovery Early Detection of Cancer Hepatology medicine.diagnostic_test business.industry Gastroenterology medicine.disease Pancreatic Neoplasms 030220 oncology & carcinogenesis Pancreatic juice Biomarker (medicine) 030211 gastroenterology & hepatology Radiology business Biomarkers Carcinoma Pancreatic Ductal |
| Zdroj: | The American journal of gastroenterology, 115(12), 2103-2108 |
| ISSN: | 1572-0241 |
| Popis: | Introduction Imaging-based surveillance programs fail to detect pancreatic ductal adenocarcinoma at a curable stage, creating an urgent need for diagnostic biomarkers. Methods Secretin-stimulated pancreatic juice (PJ) was collected from the duodenal lumen during endoscopic ultrasound. The yield of biomarkers and organoids was compared for 2 collection techniques (endoscope suction channel vs catheter-based) and 3 periods (0-4 vs 4-8 vs 8-15 minutes). Results Collection through the endoscope suction channel was superior to collection with a catheter. Collection beyond 8 minutes reduced biomarker yield. PJ-derived organoid culture was feasible. Discussion The optimal protocol for secretin-stimulated PJ collection is through the endoscope suction channel for 8 minutes allowing biomarker detection and organoid culture. |
| Databáze: | OpenAIRE |
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