The Sw5a gene confers resistance to ToLCNDV and triggers an HR response after direct AC4 effector recognition
| Autor: | Mohd Waseem, Namisha Sharma, Yusuf Khan, Supriya Chakraborty, Ashish Prasad, Mehanathan Muthamilarasan, Manoj Prasad, Pranav Pankaj Sahu, Jitendra K. Thakur |
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| Rok vydání: | 2021 |
| Předmět: |
Hypersensitive response
Genetics Multidisciplinary Effector food and beverages R gene Biology Biological Sciences Virus Up-Regulation RNA silencing MicroRNAs Solanum lycopersicum Gene Expression Regulation Plant RNA Plant Begomovirus Gene silencing Genetic Predisposition to Disease Gene Silencing Transcriptome Gene Transcription factor Plant Diseases |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 |
| Popis: | Several attempts have been made to identify antiviral genes against Tomato leaf curl New Delhi virus (ToLCNDV) and related viruses. This has led to the recognition of Ty genes (Ty1-Ty6), which have been successful in developing virus-resistant crops to some extent. Owing to the regular appearance of resistance-breaking strains of these viruses, it is important to identify genes related to resistance. In the present study, we identified a ToLCNDV resistance (R) gene, SlSw5a, in a ToLCNDV-resistant tomato cultivar, H-88-78-1, which lacks the known Ty genes. The expression of SlSw5a is controlled by the transcription factor SlMyb33, which in turn is regulated by microRNA159 (sly-miR159). Virus-induced gene silencing of either SlSw5a or SlMyb33 severely increases the disease symptoms and viral titer in leaves of resistant cultivar. Moreover, in SlMyb33-silenced plants, the relative messenger RNA level of SlSw5a was reduced, suggesting SlSw5a is downstream of the sly-miR159-SlMyb33 module. We also demonstrate that SlSw5a interacts physically with ToLCNDV-AC4 (viral suppressor of RNA silencing) to trigger a hypersensitive response (HR) and generate reactive oxygen species at infection sites to limit the spread of the virus. The "RTSK" motif in the AC4 C terminus is important for the interaction, and its mutation completely abolishes the interaction with Sw5a and HR elicitation. Overall, our research reports an R gene against ToLCNDV and establishes a connection between the upstream miR159-Myb33 module and its downstream target Sw5a to activate HR in the tomato, resulting in geminivirus resistance. |
| Databáze: | OpenAIRE |
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