Electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine A1 receptors
| Autor: | Yunchang Mo, Xiuxiu Zhuang, Junlu Wang, Wujun Geng, Jiang-Fan Chen, Qinxue Dai, He Xin, Hong-Fa Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Electroacupuncture medicine.medical_treatment adenosine triphosphate Ischemia 3-dipropylxanthine high-performance liquid chromatography neural regeneration Pharmacology Neuroprotection cerebral ischemia lcsh:RC346-429 03 medical and health sciences Adenosine A1 receptor chemistry.chemical_compound 0302 clinical medicine Developmental Neuroscience electroacupuncture medicine nerve regeneration lcsh:Neurology. Diseases of the nervous system adenosine adenosine A1 receptor pretreatment 8-cyclopentyl-1 business.industry Antagonist 8-cyclopentyl-1 3-dipropylxanthine medicine.disease Adenosine 030104 developmental biology medicine.anatomical_structure chemistry Cerebral cortex Anesthesia business Adenosine triphosphate 030217 neurology & neurosurgery Research Article medicine.drug |
| Zdroj: | Neural Regeneration Research, Vol 12, Iss 2, Pp 228-234 (2017) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| DOI: | 10.4103/1673-5374.200806 |
| Popis: | The activation of adenosine A1 receptors is important for protecting against ischemic brain injury and pretreatment with electroacupuncture has been shown to mitigate ischemic brain insult. The aim of this study was to test whether the adenosine A1 receptor mediates electroacupuncture pretreatment-induced neuroprotection against ischemic brain injury. We first performed 30 minutes of electroacupuncture pretreatment at the Baihui acupoint (GV20), delivered with a current of 1 mA, a frequency of 2/15 Hz, and a depth of 1 mm. High-performance liquid chromatography found that adenosine triphosphate and adenosine levels peaked in the cerebral cortex at 15 minutes and 120 minutes after electroacupuncture pretreatment, respectively. We further examined the effect of 15 or 120 minutes electroacupuncture treatment on ischemic brain injury in a rat middle cerebral artery-occlusion model. We found that at 24 hours reperfusion,120 minutes after electroacupuncture pretreatment, but not for 15 minutes, significantly reduced behavioral deficits and infarct volumes. Last, we demonstrated that the protective effect gained by 120 minutes after electroacupuncture treatment before ischemic injury was abolished by pretreatment with the A1-receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (1 mg/kg, intraperitoneally). Our results suggest that pretreatment with electroacupuncture at the Baihui acupoint elicits protection against transient cerebral ischemia via action at adenosine A1 receptors. |
| Databáze: | OpenAIRE |
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