Multiparametric biophysical profiling of red blood cells in malaria infection

Autor: Elizabeth S. Egan, Shreya Deshmukh, Bikash Shakya, Naside Gozde Durmus, Anna Chen, Utkan Demirci, Bryan Greenhouse
Rok vydání: 2021
Předmět:
Falciparum
0301 basic medicine
Cell type
Erythrocytes
QH301-705.5
Image Processing
Plasmodium falciparum
Biophysics
Medicine (miscellaneous)
Cell Separation
02 engineering and technology
Plasmodium falciparum infection
Biology
Article
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Computer-Assisted
Rare Diseases
Image processing
Clinical Research
Infected cell
Image Processing
Computer-Assisted
Humans
2.1 Biological and endogenous factors
Malaria
Falciparum
Aetiology
Biology (General)
equipment and supplies
021001 nanoscience & nanotechnology
Malaria
Mechanisms of disease
Good Health and Well Being
Infectious Diseases
030104 developmental biology
Isolation
separation and purification
Infection
0210 nano-technology
General Agricultural and Biological Sciences
human activities
Algorithms
Zdroj: Communications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
Communications biology, vol 4, iss 1
Communications Biology
ISSN: 2399-3642
DOI: 10.1038/s42003-021-02181-3
Popis: Biophysical separation promises label-free, less-invasive methods to manipulate the diverse properties of live cells, such as density, magnetic susceptibility, and morphological characteristics. However, some cellular changes are so minute that they are undetectable by current methods. We developed a multiparametric cell-separation approach to profile cells with simultaneously changing density and magnetic susceptibility. We demonstrated this approach with the natural biophysical phenomenon of Plasmodium falciparum infection, which modifies its host erythrocyte by simultaneously decreasing density and increasing magnetic susceptibility. Current approaches have used these properties separately to isolate later-stage infected cells, but not in combination. We present biophysical separation of infected erythrocytes by balancing gravitational and magnetic forces to differentiate infected cell stages, including early stages for the first time, using magnetic levitation. We quantified height distributions of erythrocyte populations—27 ring-stage synchronized samples and 35 uninfected controls—and quantified their unique biophysical signatures. This platform can thus enable multidimensional biophysical measurements on unique cell types.
Deshmukh et al. combine microscale magnetic levitation with minute density and magnetic susceptibility differences to enhance biophysical separation of cells. They demonstrate the feasibility of this approach on cells infected with malaria parasites, which simultaneously decrease host cell density and increase its magnetic susceptibility.
Databáze: OpenAIRE
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