Taxol production in bioreactors: Kinetics of biomass accumulation, nutrient uptake, and taxol production by cell suspensions ofTaxus baccata
| Autor: | Susan Moser, Thomas J. Hirasuna, Ronald A. Taticek, Luis J. Pestchanker, Venkatesh Srinivasan, Michael L. Shuler |
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| Rok vydání: | 1995 |
| Předmět: |
Chromatography
biology Kinetics technology industry and agriculture Biomass Bioengineering Carbohydrate equipment and supplies Phosphate biology.organism_classification complex mixtures Applied Microbiology and Biotechnology Laboratory flask chemistry.chemical_compound Nutrient chemistry Taxus Botany Bioreactor Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 47:666-676 |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.260470607 |
| Popis: | The kinetics of biomass accumulation, nutrient uptake and taxol production of Taxus baccata cell suspensions were examined in three bioreactor configurations, viz. 250-mL Erienmeyerflasks, 1-L working volume pneumatically mixed (PMB), and stirred tank (STB) bioreactors. Qualitatively similar kinetics were observed in all three bioreactor types. Biomass accumulation and specific nutrient uptake rates exhibited biphasic characteristics. Carbohydrate uptake and biomass accumulation substantially ceased when phosphate was depleted from the medium. Phosphate was identified as a possible growth-limiting nutrient. Taxol accumulated exclusively in the second phase of growth. A maximum taxol concentration of 1.5 mg/L was obtained in the PMB which was fivefold greater than that obtained in the Erienmeyer flasks and the STB, but the relative kinetics of taxol production was the same in all three reactor types. Biomass yields were calculated from the kinetic data and a stoichiometry for biomass formation was evaluated. The similarity of kinetics in the three bioreactor configurations suggests that taxol production by T. baccata cell suspensions is amenable to scateup. (c) 1995 John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
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