3D Bioprinted hydrogel model incorporating β-tricalcium phosphate for calcified cartilage tissue engineering
Autor: | Jakub Jaroszewicz, Marco Costantini, Krisztina Szöke, Marcin Heljak, Ewa Kijeńska, Alicja Kosik-Kozioł, Andrea Barbetta, Jan E. Brinchmann, Wojciech Święszkowski, Anna Mróz, Nadine Frerker, Joanna Idaszek |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Calcium Phosphates
0206 medical engineering Biomedical Engineering Bioengineering 02 engineering and technology Calcified cartilage Models Biological Biochemistry gelatin methacrylate Biomaterials Calcification Physiologic Tissue engineering medicine Humans alginate RNA Messenger Extracellular Matrix Proteins Tissue Engineering Tissue Scaffolds Viscosity Hyaline cartilage Chemistry ß-tricalcium phosphate TCP calcified cartilage Regeneration (biology) Optical Imaging Mesenchymal stem cell Temperature ALPL Hydrogels Mesenchymal Stem Cells General Medicine 021001 nanoscience & nanotechnology Chondrogenesis medicine.disease 020601 biomedical engineering Hyaline Cartilage medicine.anatomical_structure Printing Three-Dimensional coaxial needle Ink bioprinting 0210 nano-technology Biotechnology Biomedical engineering Calcification |
Popis: | One promising strategy to reconstruct osteochondral defects relies on 3D bioprinted three-zonal structures comprised of hyaline cartilage, calcified cartilage, and subchondral bone. So far, several studies have pursued the regeneration of either hyaline cartilage or bone in vitro while-despite its key role in the osteochondral region-only few of them have targeted the calcified layer. In this work, we present a 3D biomimetic hydrogel scaffold containing β-tricalcium phosphate (TCP) for engineering calcified cartilage through a co-axial needle system implemented in extrusion-based bioprinting process. After a thorough bioink optimization, we showed that 0.5% w/v TCP is the optimal concentration forming stable scaffolds with high shape fidelity and endowed with biological properties relevant for the development of calcified cartilage. In particular, we investigate the effect induced by ceramic nano-particles over the differentiation capacity of bioprinted bone marrow-derived human mesenchymal stem cells in hydrogel scaffolds cultured up to 21 d in chondrogenic media. To confirm the potential of the presented approach to generate a functional in vitro model of calcified cartilage tissue, we evaluated quantitatively gene expression of relevant chondrogenic (COL1, COL2, COL10A1, ACAN) and osteogenic (ALPL, BGLAP) gene markers by means of RT-qPCR and qualitatively by means of fluorescence immunocytochemistry. |
Databáze: | OpenAIRE |
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