3D Bioprinted hydrogel model incorporating β-tricalcium phosphate for calcified cartilage tissue engineering

Autor: Jakub Jaroszewicz, Marco Costantini, Krisztina Szöke, Marcin Heljak, Ewa Kijeńska, Alicja Kosik-Kozioł, Andrea Barbetta, Jan E. Brinchmann, Wojciech Święszkowski, Anna Mróz, Nadine Frerker, Joanna Idaszek
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Calcium Phosphates
0206 medical engineering
Biomedical Engineering
Bioengineering
02 engineering and technology
Calcified cartilage
Models
Biological
Biochemistry
gelatin methacrylate
Biomaterials
Calcification
Physiologic
Tissue engineering
medicine
Humans
alginate
RNA
Messenger
Extracellular Matrix Proteins
Tissue Engineering
Tissue Scaffolds
Viscosity
Hyaline cartilage
Chemistry
ß-tricalcium phosphate TCP
calcified cartilage
Regeneration (biology)
Optical Imaging
Mesenchymal stem cell
Temperature
ALPL
Hydrogels
Mesenchymal Stem Cells
General Medicine
021001 nanoscience & nanotechnology
Chondrogenesis
medicine.disease
020601 biomedical engineering
Hyaline Cartilage
medicine.anatomical_structure
Printing
Three-Dimensional
coaxial needle
Ink
bioprinting
0210 nano-technology
Biotechnology
Biomedical engineering
Calcification
Popis: One promising strategy to reconstruct osteochondral defects relies on 3D bioprinted three-zonal structures comprised of hyaline cartilage, calcified cartilage, and subchondral bone. So far, several studies have pursued the regeneration of either hyaline cartilage or bone in vitro while-despite its key role in the osteochondral region-only few of them have targeted the calcified layer. In this work, we present a 3D biomimetic hydrogel scaffold containing β-tricalcium phosphate (TCP) for engineering calcified cartilage through a co-axial needle system implemented in extrusion-based bioprinting process. After a thorough bioink optimization, we showed that 0.5% w/v TCP is the optimal concentration forming stable scaffolds with high shape fidelity and endowed with biological properties relevant for the development of calcified cartilage. In particular, we investigate the effect induced by ceramic nano-particles over the differentiation capacity of bioprinted bone marrow-derived human mesenchymal stem cells in hydrogel scaffolds cultured up to 21 d in chondrogenic media. To confirm the potential of the presented approach to generate a functional in vitro model of calcified cartilage tissue, we evaluated quantitatively gene expression of relevant chondrogenic (COL1, COL2, COL10A1, ACAN) and osteogenic (ALPL, BGLAP) gene markers by means of RT-qPCR and qualitatively by means of fluorescence immunocytochemistry.
Databáze: OpenAIRE
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