Mouse model of ocular hypertension with retinal ganglion cell degeneration
| Autor: | Eiichi Hasegawa, Ryo Mukai, Meredith S Gregory-Ksander, Kip M. Connor, Yoko Okunuki, Anitha Krishnan, Garrett Klokman, Dong Ho Park, Joan W. Miller, Deeba Husain, Clifford Kim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Retinal Ganglion Cells 0301 basic medicine Intraocular pressure Eye Diseases genetic structures Polymers Ocular hypertension Glaucoma Blood Pressure Degeneration (medical) Vascular Medicine Cornea Mice 0302 clinical medicine Animal Cells Medicine and Health Sciences Materials Neurons Multidisciplinary Retinal Degeneration Immunohistochemistry Chemistry medicine.anatomical_structure Macromolecules Retinal ganglion cell Physical Sciences Hypertension Medicine Cellular Types Anatomy Tomography Optical Coherence Research Article medicine.medical_specialty Programmed cell death Ganglion Cells Science Ocular Anatomy Materials Science Retina Microbeads 03 medical and health sciences Ocular System Ophthalmology medicine Animals Intraocular Pressure business.industry Biology and Life Sciences Afferent Neurons Cell Biology Polymer Chemistry medicine.disease eye diseases Mice Inbred C57BL Disease Models Animal 030104 developmental biology Cellular Neuroscience 030221 ophthalmology & optometry Eyes Ocular Hypertension sense organs business Head Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 1, p e0208713 (2019) |
| ISSN: | 1932-6203 |
| Popis: | ObjectivesOcular hypertension is a primary risk factor for glaucoma and results in retinal ganglion cell (RGC) degeneration. Current animal models of glaucoma lack severe RGC cell death as seen in glaucoma, making assessment of physiological mediators of cell death difficult. We developed a modified mouse model of ocular hypertension whereby long-lasting elevation of intraocular pressure (IOP) is achieved, resulting in significant reproducible damage to RGCs.ResultsIn this model, microbeads are mixed with hyaluronic acid and injected into the anterior chamber of C57BL/6J mice. The hyaluronic acid allows for a gradual release of microbeads, resulting in sustained blockage of Schlemm's canal. IOP elevation was bimodal during the course of the model's progression. The first peak occurred 1 hours after beads injection, with an IOP value of 44.69 ± 6.00 mmHg, and the second peak occurred 6-12 days post-induction, with an IOP value of 34.91 ± 5.21 mmHg. RGC damage was most severe in the peripheral retina, with a loss of 64.1% compared to that of untreated eyes, while the midperiphery exhibited a 32.4% loss, 4 weeks following disease induction.ConclusionsThese results suggest that sustained IOP elevation causes more RGC damage in the periphery than in the midperiphery of the retina. This model yields significant and reproducible RGC degeneration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|