Different genotoxic profiles between depleted and enriched uranium

Autor: Sandrine Frelon, M. De Meo, Isabelle Dublineau, F. Petitot, D. Broggio, A. Feugier, Carine Darolles
Přispěvatelé: PRP-HOM/SRBE/LRTOX, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Laboratoire d'évaluation de la dose interne (DRPH/SDI/LEDI), Biogénotoxicologie et Mutagenèse Environnementale (BME), Université de la Méditerranée - Aix-Marseille 2, Laboratoire de radiotoxicologie et radiobiologie expérimentale (LRTOX), Laboratoire de radiotoxicologie et radiobiologie expérimentale (IRSN/PRP-HOM/SRBE/LRTOX), Laboratoire d'évaluation de la dose interne (IRSN/DRPH/SDI/LEDI), Service de Dosimétrie Interne (IRSN/DRPH/SDI), Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Laboratoire de radiotoxicologie et radiobiologie expérimentale (IRSN/PSE-SANTE/SESANE/LRTOX), Service de recherche sur les effets biologiques et Sanitaires des rayonnements ionisants (IRSN/PSE-SANTE/SESANE)
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Binucleated cells
[SDV]Life Sciences [q-bio]
uranium 235
radiation exposure
animal cell
Toxicology
medicine.disease_cause
fibroblast
Mice
0302 clinical medicine
Immunologic
Depleted uranium
Cells
Cultured
In Situ Hybridization
Fluorescence
In Situ Hybridization
Cytotoxicity Tests
0303 health sciences
Mice
Inbred C3H
Cultured
Radiation
Micronucleus Tests
Cell Death
Chemistry
article
radiation carcinogenesis
risk assessment
General Medicine
Uranium
Inbred C3H
micronucleus test
priority journal
centromere
030220 oncology & carcinogenesis
Micronucleus test
Uranium-235
Drug
ionizing radiation
Monte Carlo Method
Cell Division
Cells
chemistry.chemical_element
embryo
cytokinesis
Enriched uranium
complex mixtures
Fluorescence
Dose-Response Relationship
03 medical and health sciences
medicine
Animals
controlled study
mouse
030304 developmental biology
nonhuman
Dose-Response Relationship
Drug
concentration (parameters)
Radiochemistry
genotoxicity
Dose-Response Relationship
Radiation
Cytotoxicity Tests
Immunologic
Micronucleus
Genotoxicity
Mutagens
Zdroj: Toxicology Letters
Toxicology Letters, 2010, 192 (3), pp.337-348. ⟨10.1016/j.toxlet.2009.11.009⟩
ISSN: 0378-4274
1879-3169
DOI: 10.1016/j.toxlet.2009.11.009⟩
Popis: Uranium is an alpha-particle-emitting heavy metal. Its genotoxicity results from both its chemical and its radiological properties that vary with its isotopic composition (12% enriched uranium in 235U (EU) has a specific activity 20 times higher than 0.3% depleted uranium in 235U (DU)). The influence of the isotopic composition of uranium on its genotoxic profile (clastogenic/aneugenic) has never been described. The present study evaluated genotoxic profile of uranium with the cytokinesis-block micronucleus centromere assay. C3H10T1/2 mouse embryo fibroblasts were contaminated with either DU or EU at different concentrations (5 μM, 50 μM and 500 μM). Cells received low doses ranging from 0.3 μGy to 760.5 μGy. The frequency of binucleated cells with one micronucleus increased with increasing concentrations of both DU and EU in the same way. EU induced more centromere-negative micronuclei and nucleoplasmic bridges than DU. A correlation between these two clastogenic markers and ionizing radiation doses was observed. Finally, this study showed that the genotoxic profile of uranium depends on its isotopic composition. DU and EU are low and high clastogens, respectively. However, DU aneugenic effects remain high. Thus, there is a need to study the potential role of aneugenic effects of DU in carcinogenic risk assessment linked to uranium internal exposure. © 2009 Elsevier Ireland Ltd. All rights reserved.
Databáze: OpenAIRE
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