Crystal structure of the high-alkaline serine protease PB92 from Bacillus alcalophilus
| Autor: | O. Misset, Bauke W. Dijkstra, J.M. van der Laan, Leonardus Johannes Sofie Marie Mulleners, H. Kelders, Kor H. Kalk, Alexey Teplyakov |
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| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology |
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Models
Molecular Stereochemistry Protein Conformation medicine.medical_treatment EGLIN-C Molecular Sequence Data Bioengineering Bacillus LEAST-SQUARES REFINEMENT Biochemistry X-RAY STRUCTURE ALKALINE PROTEASE INHIBITOR COMPLEXES X-Ray Diffraction Bacillus alcalophilus medicine Molecular replacement BACILLUS-ALCALOPHILUS Amino Acid Sequence Subtilisins Molecular Biology Protein secondary structure 3-DIMENSIONAL STRUCTURE Serine protease Protease SERINE PROTEASE biology Chemistry Serine Endopeptidases fungi Subtilisin Hydrogen-Ion Concentration Proteinase K biology.organism_classification SUBTILISIN CARLSBERG AMYLOLIQUEFACIENS SUBTILISIN Crystallography RESOLUTION PROTEINASE-K biology.protein X-RAY MOLECULAR-STRUCTURE Biotechnology |
| Zdroj: | Protein Engineering, 5(5), 405-411 |
| ISSN: | 0269-2139 |
| Popis: | The crystal structure of a serine protease from the alkalophilic strain Bacillus alcalophilus PB92 has been determined by X-ray diffraction at 1.75 A resolution. The structure has been solved by molecular replacement using the atomic model of subtilisin Carlsberg. The model of the PB92 protease has been refined to an R-factor of 14.0% and contains 1882 protein atoms, two calcium ions and 188 water molecules. The overall folding of the polypeptide chain closely resembles that of the subtilisins. Furthermore, almost all of the secondary structure elements found in subtilisin Carlsberg are also present in the PB92 protease. The major differences between the two structures are located around the deletion regions (residues 37 and 158-161 in subtilisin Carlsberg) and in two loops which are known to be the most variable parts of subtilisin structures. Flexibility of one of these loops (residues 126-130 in the PB92 protease) is believed to account for the induced-fit mechanism of substrate binding. |
| Databáze: | OpenAIRE |
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