Distinct developmental subtypes of cultured non-stellate rat astrocytes distinguished by a new glial intermediate filament-associated protein
| Autor: | George D. Pappas, Hsi Yuang Yang, Robert D. Goldman, Norman Lieska, Diane Johnson-Seaton |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Blotting Western Central nervous system Fluorescent Antibody Technique Vimentin Immunofluorescence Pregnancy Glial Fibrillary Acidic Protein medicine Animals Intermediate filament Molecular Biology Cells Cultured Cerebral Cortex Glial fibrillary acidic protein biology medicine.diagnostic_test General Neuroscience Antibodies Monoclonal Rats Inbred Strains Rats Cell biology medicine.anatomical_structure Microscopy Fluorescence Cerebral cortex Astrocytes biology.protein Neuroglia Electrophoresis Polyacrylamide Gel Female Neurology (clinical) Neuroscience Biomarkers Developmental Biology Astrocyte |
| Zdroj: | Brain Research. 573:161-168 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(92)90127-u |
| Popis: | The nature and tissue origin of cultured non-stellate astrocytes have not been defined. On the basis of immunofluorescence microscopy using multiple double-labeling with antibodies to glial fibrillary acidic protein (GFAP), vimentin, and the recently identified intermediate filament-associated protein (IFAP)-70/280kD, four distinct astrocytic subtypes were definable in neonatal rat brain astrocytes in culture. All of these were of the non-stellate type on the basis of morphology. Similar examination of developing rat cerebral cortex identified these same subtypes as distinct differentiation states of astrocytes. These findings indicate that the parallel developmental events can be studied in vitro. |
| Databáze: | OpenAIRE |
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