An ancient RAB5 governs the formation of additional vacuoles and cell shape in petunia petals
| Autor: | Martina Cerri, Mattijs Bliek, Francesca Quattrocchio, Lara Reale, Enric Martínez-Calvó, Yanbang Li, Biao Lai, Ronald Koes, Michiel Vandenbussche, Pamela Strazzer, Shuangjiang Li, Cornelis Spelt |
|---|---|
| Přispěvatelé: | University of Amsterdam [Amsterdam] (UvA), Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Plant Development & (Epi)Genetics (SILS, FNWI), Faculty of Science, SILS Other Research (FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
RAB GTPase Endosome QH301-705.5 [SDV]Life Sciences [q-bio] Cell Arabidopsis vacuolino Endosomes Vacuole 01 natural sciences Petunia General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Gene duplication medicine Animals petunia Biology (General) Cell Shape Transcription factor rab5 GTP-Binding Proteins 030304 developmental biology 0303 health sciences biology vacuole Arabidopsis Proteins Chemistry biology.organism_classification Cell biology flower Protein Transport medicine.anatomical_structure endosome trafficking rab GTP-Binding Proteins conical cell shape MBW complex Vacuoles Petal Neofunctionalization Lysosomes 010606 plant biology & botany |
| Zdroj: | Cell Reports Cell Reports, Elsevier Inc, 2021, 36 (13), pp.109749. ⟨10.1016/j.celrep.2021.109749⟩ Cell Reports, 2021, 36 (13), pp.109749. ⟨10.1016/j.celrep.2021.109749⟩ Cell Reports, 36(13):109749. Cell Press Cell Reports, Vol 36, Iss 13, Pp 109749-(2021) |
| ISSN: | 2211-1247 2639-1856 |
| Popis: | International audience; Homologous ("canonical'') RAB5 proteins regulate endosomal trafficking to lysosomes in animals and to the central vacuole in plants. Epidermal petal cells contain small vacuoles (vacuolinos) that serve as intermediate stations for proteins on their way to the central vacuole. Here, we show that transcription factors required for vacuolino formation in petunia induce expression of RAB5a. RAB5a defines a previously unrecognized clade of canonical RAB5s that is evolutionarily and functionally distinct from ARA7-type RAB5s, which act in trafficking to the vacuole. Loss of RAB5a reduces cell height and abolishes vacuolino formation, which cannot be rescued by the ARA7 homologs, whereas constitutive RAB5a (over)expression alters the conical cell shape and promotes homotypic vacuolino fusion, resulting in oversized vacuolinos. These findings provide a rare example of how gene duplication and neofunctionalization increased the complexity ofmembrane trafficking during evolution and suggest a mechanism by which cells may form multiple vacuoles with distinct content and function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|