Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast
| Autor: | William T. Molin, Mark Blenner, Christopher A. Saski, Allison Yaguchi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
DNA Replication
0301 basic medicine DNA Plant Sequence analysis Autonomously replicating sequence lcsh:Medicine Saccharomyces cerevisiae General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Autonomous replication Replication (statistics) Replicon Cloning Molecular lcsh:Science (General) lcsh:QH301-705.5 Sequence (medicine) Genetics Base Composition Amaranthus DNA bending biology lcsh:R EACS Sequence Analysis DNA General Medicine biology.organism_classification eccDNA replicon Yeast Amaranthus palmeri Research Note 030104 developmental biology lcsh:Biology (General) biology.protein Nucleic Acid Conformation DNA unwinding element 030217 neurology & neurosurgery lcsh:Q1-390 |
| Zdroj: | BMC Research Notes, Vol 13, Iss 1, Pp 1-6 (2020) BMC Research Notes |
| DOI: | 10.21203/rs.3.rs-29210/v2 |
| Popis: | Objective The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. Results Sequence analysis of the eccDNA replicon revealed a region of sharp changes in A + T/G + C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid. |
| Databáze: | OpenAIRE |
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