Brain transcriptome changes in the aging Drosophila melanogaster accompany olfactory memory performance deficits

Autor: Erica Walkinshaw, Courtney M. MacMullen, Rodrigo Pacifico, Ronald L. Davis, Xiaofan Zhang
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Aging
Physiology
Gene Expression
Biochemistry
Oxidative Phosphorylation
Transcriptome
Cognition
Learning and Memory
0302 clinical medicine
Gene expression
Medicine and Health Sciences
Drosophila Proteins
Gene Regulatory Networks
Cognitive decline
Energy-Producing Organelles
Cognitive Impairment
Multidisciplinary
biology
Cognitive Neurology
Drosophila Melanogaster
Age Factors
Brain
Eukaryota
Genomics
Animal Models
Mitochondria
Insects
Experimental Organism Systems
Neurology
Models
Animal
Medicine
Female
Drosophila
Cellular Structures and Organelles
Drosophila melanogaster
Transcriptome Analysis
Research Article
Arthropoda
Cognitive Neuroscience
Science
Oxidative phosphorylation
Bioenergetics
Research and Analysis Methods
03 medical and health sciences
Sex Factors
Model Organisms
Memory
Genetics
Animals
Cognitive Dysfunction
Olfactory memory
Gene
Sequence Analysis
RNA
Gene Expression Profiling
Electron Transport Chain
Organisms
Biology and Life Sciences
Computational Biology
Cell Biology
Olfactory Perception
Genome Analysis
biology.organism_classification
Invertebrates
Gene expression profiling
030104 developmental biology
Animal Studies
Cognitive Science
Physiological Processes
Organism Development
Neuroscience
030217 neurology & neurosurgery
Developmental Biology
Zdroj: PLoS ONE, Vol 13, Iss 12, p e0209405 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Cognitive decline is a common occurrence of the natural aging process in animals and studying age-related changes in gene expression in the brain might shed light on disrupted molecular pathways that play a role in this decline. The fruit fly is a useful neurobiological model for studying aging due to its short generational time and relatively small brain size. We investigated age-dependent changes in the Drosophila melanogaster whole-brain transcriptome by comparing 5-, 20-, 30- and 40-day-old flies of both sexes. We used RNA-Sequencing of dissected brain samples followed by differential expression, temporal clustering, co-expression network and gene ontology enrichment analyses. We found an overall decline in expression of genes from the mitochondrial oxidative phosphorylation pathway that occurred as part of aging. We also detected, in females, a pattern of continuously declining expression for many neuronal function genes, which was unexpectedly reversed later in life. This group of genes was highly enriched in memory-impairing genes previously identified through an RNAi screen. We also identified deficits in short-term olfactory memory performance in older flies of both sexes, some of which matched the timing of certain changes in the brain transcriptome. Our study provides the first transcriptome profile of aging brains from fruit flies of both sexes, and it will serve as an important resource for those who study aging and cognitive decline in this model.
Databáze: OpenAIRE
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