Dysregulated LIF-STAT3 pathway is responsible for impaired embryo implantation in a Streptozotocin-induced diabetic mouse model
| Autor: | Fu-Niu Qin, Fei Gao, Qian-Rong Qi, Tong-Song Wang, Ru-Juan Zuo, Ji-Long Liu, Zi-Long Li, Zeng-Ming Yang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
QH301-705.5 medicine.drug_class Science medicine.medical_treatment General Biochemistry Genetics and Molecular Biology Internal medicine Diabetes mellitus medicine Insulin Biology (General) STAT3 Type 1 diabetes LIF-STAT3 biology business.industry Diabetes Embryo Streptozotocin medicine.disease Estrogen Embryo transfer Endocrinology Embryo implantation embryonic structures biology.protein General Agricultural and Biological Sciences business hormones hormone substitutes and hormone antagonists Research Article medicine.drug |
| Zdroj: | Biology Open Biology Open, Vol 4, Iss 7, Pp 893-902 (2015) |
| ISSN: | 2046-6390 |
| DOI: | 10.1242/bio.011890 |
| Popis: | The prevalence of diabetes is increasing worldwide with the trend of patients being young and creating a significant burden on health systems, including reproductive problems, but the effects of diabetes on embryo implantation are still poorly understood. Our study was to examine effects of diabetes on mouse embryo implantation, providing experimental basis for treating diabetes and its complications. Streptozotocin (STZ) was applied to induce type 1 diabetes from day 2 of pregnancy or pseudopregnancy in mice. Embryo transfer was used to analyze effects of uterine environment on embryo implantation. Our results revealed that the implantation rate is significantly reduced in diabetic mice compared to controls, and the change of uterine environment is the main reason leading to the decreased implantation rate. Compared to control, the levels of LIF and p-STAT3 are significantly decreased in diabetic mice on day 4 of pregnancy, and serum estrogen level is significantly higher. Estrogen stimulates LIF expression under physiological level, but the excessive estrogen inhibits LIF expression. LIF, progesterone or insulin supplement can rescue embryo implantation in diabetic mice. Our data indicated that the dysregulated LIF-STAT3 pathway caused by the high level of estrogen results in the impaired implantation in diabetic mice, which can be rescued by LIF, progesterone or insulin supplement. |
| Databáze: | OpenAIRE |
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