Evaluation of a commercial enzyme-linked immunosorbent assay (ELISA) for detecting antibodies against Toxoplasma gondii from naturally and experimentally infected pigs

Autor: Carmelo Russo, Francesca Gucciardi, Francesco Mira, Anna Maria Fausta Marino, Giusi Macaluso, Santina Di Bella, Giuseppa Purpari, Annalisa Guercio, Elisabetta Giudice, Maria Angeles Gómez-Morales
Rok vydání: 2018
Předmět:
0301 basic medicine
Microbiology (medical)
Immunology and Microbiology (all)
Swine
030106 microbiology
Toxoplasma gondii
Antibodies
Protozoan
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
Antibodies
03 medical and health sciences
Elisa kit
0302 clinical medicine
parasitic diseases
Animals
Medicine
030212 general & internal medicine
Direct fluorescent antibody
Swine Diseases
chemistry.chemical_classification
Kappa value
General Immunology and Microbiology
biology
business.industry
Incidence
pigs
General Medicine
Antibodies
ELISA
IFAT
pigs
Toxoplasma gondii
Immunology and Microbiology (all)
Microbiology (medical)
Infectious Diseases
medicine.disease
biology.organism_classification
IFAT
Virology
Toxoplasmosis
Specific antibody
Toxoplasmosis
Animal
Infectious Diseases
Enzyme
ROC Curve
chemistry
biology.protein
ELISA
Antibody
business
Toxoplasma
Switzerland
Zdroj: Infectious Diseases. 51:26-31
ISSN: 2374-4243
2374-4235
DOI: 10.1080/23744235.2018.1490026
Popis: Background Toxoplasmosis is one of the most frequent parasitic infections in animals causing reproductive disorders and thus notable economic losses in productivity. Among food animals, pigs along with sheep and goats possess the highest incidence of Toxoplasma gondii cysts in meat, and play a role as a source of human infection. Methods The commercial ELISA kit (PrioCHECK® Toxoplasma Ab SR, Prionics Schlieren-Zurich, Switzerland) for the detection of specific antibodies against T. gondii in swine serum was compared with a commercial IFAT (indirect fluorescent antibody test) (Toxo-Spot IF, bioMerieux, France), used as the reference test. Results The kappa value obtained comparing the results performed on sera by ELISA with the by IFAT was 1. By a receiver operating characteristics curve analysis, the commercial ELISA had a relative sensitivity of 100%, and a relative specificity of 100% respect to IFAT. Conclusions The commercial ELISA showed a very good agreement with the commercial IFAT in the detection of serum antibodies to Toxoplasma in pigs. Our study confirmed the usefulness of the commercial ELISA kit for the detection of antibodies against T. gondii in pigs, representing a valuable tool to improve the diagnostic activity for T. gondii in swine populations at the farm level or at the slaughterhouse, contributing to the control of this widespread infection.
Databáze: OpenAIRE
Externí odkaz: