Micronucleus test with vincristine sulfate and colchicine in peripheral blood reticulocytes of mice using acridine orange supravital staining
| Autor: | Inoue Kazuyoshi, Nakajima Madoka, Nito Shinji, Kondo Yasushi, Miyahana Koichi, Hayashi Mikiko, Honda Sachiko, Shinagawa Yasuhiro, Sato Shigeaki, Ariyuki Fumio |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Vincristine Vincristine Sulfate Reticulocytes Mice Inbred Strains Toxicology chemistry.chemical_compound Mice Reticulocyte Genetics medicine Colchicine Animals Micronucleus Tests Dose-Response Relationship Drug Acridine orange Molecular biology Acridine Orange Supravital staining medicine.anatomical_structure chemistry Immunology Micronucleus test Micronucleus medicine.drug Mutagens |
| Zdroj: | Mutation research. 278(2-3) |
| ISSN: | 0027-5107 |
| Popis: | The induction of micronuclei in mouse peripheral blood reticulocytes (RETs) was studied with the spindle poisons vincristine sulfate (VINO and colchicine (COL) using acridine orange (AO) supravital staining. Each chemical was studied independently in two laboratories using the same protocol. Blood samples were prepared at 0, 24, 48, and 72 h after a single intraperitoneal treatment with VINC (0.0625, 0.125, and 0.25 mg/kg) or COL (0.25, 0.5, 1.0, and 2.0 mg/kg). Both VINC and COL induced micronucleated RETs (MNRETs) significantly and dose-dependently with a peak at 48 h after treatment. Maximum frequencies of micronucleated polychromatic erythrocytes (MNPCEs) were observed 24 h after treatment with VINC; thus, the transition time from MNPCEs to MNRETs was about 24 h. Both spindle poisons gave comparable results in the paired laboratories, indicating that the present AO supravital staining method is highly reproducible. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|