Simultaneous phenotyping of leaf growth and chlorophyll fluorescence via GROWSCREEN FLUORO allows detection of stress tolerance in Arabidopsis thaliana and other rosette plants
| Autor: | Silvia Braun, Marcus Jansen, Michael Metzlaff, Frank Gilmer, Achim Walter, Sabine Briem, Hanno Scharr, Andreas Fischbach, Georg Dreissen, Kerstin A. Nagel, Uwe Rascher, Bernhard Biskup, Ulrich Schurr, Susanne Tittmann, Iris De Jaeger |
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| Rok vydání: | 2009 |
| Předmět: |
Ecophysiology
biology Abiotic stress Nicotiana tabacum fungi food and beverages Plant Science biology.organism_classification Photosynthesis chemistry.chemical_compound chemistry Chlorophyll Botany Biophysics Arabidopsis thaliana Agronomy and Crop Science Developmental biology Chlorophyll fluorescence |
| Zdroj: | Functional plant biology : FPB. 36(11) |
| ISSN: | 1445-4416 |
| Popis: | Stress caused by environmental factors evokes dynamic changes in plant phenotypes. In this study, we deciphered simultaneously the reaction of plant growth and chlorophyll fluorescence related parameters using a novel approach which combines existing imaging technologies (GROWSCREEN FLUORO). Three different abiotic stress situations were investigated demonstrating the benefit of this approach to distinguish between effects related to (1) growth, (2) chlorophyll-fluorescence, or (3) both of these aspects of the phenotype. In a drought stress experiment with more than 500 plants, poly(ADP-ribose) polymerase (PARP) deficient lines of Arabidopsis thaliana (L.) Heynh showed increased relative growth rates (RGR) compared with C24 wild-type plants. In chilling stress, growth of PARP and C24 lines decreased rapidly, followed by a decrease in Fv/Fm. Here, PARP-plants showed a more pronounced decrease of Fv/Fm than C24, which can be interpreted as a more efficient strategy for survival in mild chilling stress. Finally, the reaction of Nicotiana tabacum L. to altered spectral composition of the intercepted light was monitored as an example of a moderate stress situation that affects chlorophyll-fluorescence related, but not growth-related parameters. The examples investigated in this study show the capacity for improved plant phenotyping based on an automated and simultaneous evaluation of growth and photosynthesis at high throughput. |
| Databáze: | OpenAIRE |
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