Beneficial Roles of Cellulose Patch-Mediated Cell Therapy in Myocardial Infarction: A Preclinical Study
| Autor: | Daiany Souza, Julio Cesar Francisco, Clayton F. de Souza, Rossana Baggio Simeoni, Katherine Athayde Teixeira de Carvalho, Maria Rita Sierakowski, Luiz Cesar Guarita-Souza, Célia Regina Cavichiolo Franco, Eltyeb Abdelwaid, Nelson Itiro Miyague, Carolina Maria Costa de Oliveira Souza, Priscila Elias Ferreira Stricker, Ana Carolina Irioda, Nádia Nascimento da Rosa, Bassam Felipe Mogharbel |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty implant Cell- and Tissue-Based Therapy Neovascularization Physiologic 02 engineering and technology Anterior Descending Coronary Artery Ventricular Function Left Article Cell therapy 03 medical and health sciences Keywords: implant Patch formation Internal medicine medicine Animals bacterial cellulose patch Myocardial infarction Rats Wistar Cellulose lcsh:QH301-705.5 Ejection fraction Ventricular Remodeling business.industry Myocardium Heart Stroke Volume General Medicine 021001 nanoscience & nanotechnology medicine.disease 030104 developmental biology myocardial infarction lcsh:Biology (General) Cardiology Implant cell therapy delivery 0210 nano-technology business Ischemic heart |
| Zdroj: | Cells Volume 10 Issue 2 Cells, Vol 10, Iss 424, p 424 (2021) |
| ISSN: | 2073-4409 |
| Popis: | Biological scaffolds have become an attractive approach for repairing the infarcted myocardium and have been shown to facilitate constructive remodeling in injured tissues. This study aimed to investigate the possible utilization of bacterial cellulose (BC) membrane patches containing cocultured cells to limit myocardial postinfarction pathology. Myocardial infarction (MI) was induced by ligating the left anterior descending coronary artery in 45 Wistar rats, and patches with or without cells were attached to the hearts. After one week, the animals underwent echocardiography to assess for ejection fraction and left ventricular end-diastolic and end-systolic volumes. Following patch formation, the cocultured cells retained viability of > 90% over 14 days in culture. The patch was applied to the myocardial surface of the infarcted area after staying 14 days in culture. Interestingly, the BC membrane without cellular treatment showed higher preservation of cardiac dimensions however, we did not observe improvement in the left ventricular ejection fraction of this group compared to coculture-treated membranes. Our results demonstrated an important role for BC in supporting cells known to produce cardioprotective soluble factors and may thus provide effective future therapeutic outcomes for patients suffering from ischemic heart disease. |
| Databáze: | OpenAIRE |
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