2,4-Dinitrophenol pharmacologically promotes retinal detachment in rabbits
| Autor: | Ahad Deen, Amish Purohit, Scott M. Steidl, Karen Bassichis-Saland, Jeffrey D. Benner, Theresa Kramer |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
genetic structures medicine.medical_treatment Macular translocation Vitrectomy Retina 2 4-Dinitrophenol Injections chemistry.chemical_compound Ophthalmology medicine Animals Focal Adhesions business.industry Uncoupling Agents Retinal Detachment Retinal detachment Retinal General Medicine medicine.disease eye diseases Retinal toxicity medicine.anatomical_structure chemistry Toxicity sense organs Rabbits business |
| Zdroj: | Retina (Philadelphia, Pa.). 25(3) |
| ISSN: | 0275-004X |
| Popis: | Purpose The most difficult and unpredictable step of macular translocation surgery is creating the retinal detachment. The authors evaluated the efficacy of 2,4-dinitrophenol (2,4-DNP) to promote retinal detachment in the rabbit. Methods A vitrectomy was performed in each eye of a Dutch-belted rabbit. One eye was injected with 0.1 cc of a 5 mmol/L 2,4-DNP, the other eye with 0.1 cc of BSS+. After 30 minutes, the minimum aspiration pressure required to visibly elevate the retina was recorded. Four nonvitrectomized eyes received an intravitreal injection of either 0.1 cc of BSS+ or 5 mmol/L 2,4-DNP, and were enucleated and fixated for histopathologic examination. Results In the 12 masked eyes, the mean aspiration pressure decreased from 217 +/- 20 mmHg in the six BSS+ eyes to 117 +/- 20 mmHg in the six 2,4-DNP treated eyes (P = 0.0022). A retinal detachment was present in three of six masked and two of two unmasked 2,4-DNP treated eyes and none of eight BSS+ treated eyes. There was no short-term toxicity to the retina at the light microscope level. Conclusion Intravitreal injection of 2,4-DNP reduced the retinal adhesive force by over 50% when compared to the BSS+ treated control eyes, without any short-term retinal toxicity. |
| Databáze: | OpenAIRE |
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