Dynamic imaging and quantitative analysis of cranial neural tube closure in the mouse embryo using optical coherence tomography
Autor: | Irina V. Larina, Monica D. Garcia, Andrew L. Lopez, Shang Wang, Paul A. Overbeek, Kirill V. Larin |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Neural tube defect medicine.diagnostic_test Dynamic imaging Embryogenesis Neural tube Hindbrain Anatomy Biology medicine.disease Atomic and Molecular Physics and Optics Article Midbrain 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Optical coherence tomography Live cell imaging medicine Biotechnology |
Zdroj: | Biomedical optics express. 8(1) |
ISSN: | 2156-7085 |
Popis: | Neural tube closure is a critical feature of central nervous system morphogenesis during embryonic development. Failure of this process leads to neural tube defects, one of the most common forms of human congenital defects. Although molecular and genetic studies in model organisms have provided insights into the genes and proteins that are required for normal neural tube development, complications associated with live imaging of neural tube closure in mammals limit efficient morphological analyses. Here, we report the use of optical coherence tomography (OCT) for dynamic imaging and quantitative assessment of cranial neural tube closure in live mouse embryos in culture. Through time-lapse imaging, we captured two neural tube closure mechanisms in different cranial regions, zipper-like closure of the hindbrain region and button-like closure of the midbrain region. We also used OCT imaging for phenotypic characterization of a neural tube defect in a mouse mutant. These results suggest that the described approach is a useful tool for live dynamic analysis of normal neural tube closure and neural tube defects in the mouse model. |
Databáze: | OpenAIRE |
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