Effect of Berberine on Interleukin 8 and Monocyte Chemotactic Protein 1 Expression in a Human Retinal Pigment Epithelial Cell Line
| Autor: | Hu-Shan Cui, Zai-Long Chi, Lian-Shun Zheng, Yoriko Hayasaka, Xue-Yun Zhang, Seiji Hayasaka |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chemokine
Berberine Gene Expression Enzyme-Linked Immunosorbent Assay Biology Cell Line Cellular and Molecular Neuroscience chemistry.chemical_compound Gene expression Humans RNA Messenger Interleukin 8 Pigment Epithelium of Eye Chemokine CCL2 Messenger RNA Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Interleukin-8 Interleukin General Medicine Molecular biology Sensory Systems Ophthalmology chemistry Cell culture biology.protein RNA Tumor necrosis factor alpha Interleukin-1 |
| Zdroj: | Ophthalmic Research. 38:149-157 |
| ISSN: | 1423-0259 0030-3747 |
| DOI: | 10.1159/000090672 |
| Popis: | Purpose: The aims of this study were to examine the effects of berberine, an alkaloid isolated from some medicinal herbs, on interleukin 8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) expression in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin 1β (IL-1β) or tumor necrosis factor α (TNF-α). Methods: ARPE-19 cells were cultured to confluence. Berberine and IL-1β or TNF-α were added to the medium. IL-8 mRNA and MCP-1 mRNA were measured by semiquantitative reverse-transcription polymerase chain reaction and real-time polymerase chain reaction. IL-8 and MCP-1 protein concentrations in the media were measured using enzyme-linked immunosorbent assay. Results: Berberine dose-dependently inhibited IL-8 mRNA and MCP-1 mRNA expression of the cells and protein levels in the media stimulated with IL-1β or TNF-α. Conclusion: These findings indicate that berberine dose-dependently inhibited the expression of IL-8 and MCP-1 induced by IL-1β or TNF-α. |
| Databáze: | OpenAIRE |
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