Trisialoganglioside synthesis by a chicken brain sialyltransferase. Comparative study with the similar reaction for the synthesis of disialoganglioside
| Autor: | Ranwel Caputto, F. A. Cumar, M. G. Mestrallet |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Aging
Sialyltransferase Clinical Biochemistry Polysorbates Chick Embryo macromolecular substances Polyethylene Glycols Catalysis Structure-Activity Relationship chemistry.chemical_compound Transferases Gangliosides Animals Molecular Biology chemistry.chemical_classification biology Brain food and beverages Embryo Cell Biology General Medicine Hydrogen-Ion Concentration Molecular biology Sialyltransferases Sialic acid carbohydrates (lipids) Kinetics Trisialoganglioside Subcellular distribution Enzyme chemistry Biochemistry Galactose biology.protein Cattle lipids (amino acids peptides and proteins) Chickens Subcellular Fractions |
| Zdroj: | Molecular and Cellular Biochemistry. 16:63-70 |
| ISSN: | 1573-4919 0300-8177 |
| DOI: | 10.1007/bf01732045 |
| Popis: | An enzyme preparation from embryonic chicken brain catalyzes the transfer of sialic acid from CMP-N-acetylneuraminic acid to ceramide-Glc-Gal(NeuAc-NeuAc)-GalNAc-Gal (GDlb) to form ceramide-Glc-Gal(NeuAc-NeuAc)-GalNAc-Gal-NeuAc (GTlb). The sialyltransferase activity was measured during the development of the embryo, the subcellular distribution of this activity was determined and several kinetic properties of the reaction were ex-amined. A comparative study with the similar reaction involved in the transfer of sialic acid to the terminal galactose in ceramide-Glc-Gal(NeuAc)-GalNAc-Gal (GMl) was made. The results obtained in this comparative study suggest that the transfer of sialic acid in both reactions is catalyzed by the same enzyme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink