Inter-retrotransposon-amplified polymorphism markers for germplasm characterization in Manihot esculenta (Euphorbiaceae)
| Autor: | Charles R. Clement, Nelcimar Reis Sousa, Gilvan Ferreira da Silva, A.M. Oliveira-Silva, M. C. Dias |
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| Rok vydání: | 2014 |
| Předmět: |
Germplasm
Manihot DNA Plant Genotype Retroelements Microsatellite Dna Retrotransposon Biology Long Terminal Repeat Genome Germ Cell Loss of heterozygosity Polymorphism (computer science) Genetics Controlled Study Genetic variability Molecular Biology Cassava Genetic diversity Polymorphism Genetic Retroposon Dna Polymorphism fungi Gene Amplification Genetic Variation food and beverages Primer Dna General Medicine Nonhuman Long terminal repeat Germ Cells Genetic Variability Genetic Database Dna Plant Shannon Information Index Marker Gene Microsatellite Repeats |
| Zdroj: | Repositório Institucional do INPA Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/2014.may.16.3 |
| Popis: | Manioc, Manihot esculenta, is economically important in many tropical and subtropical countries. The genetic variability of the species has not been fully explored, and new information may help expand its use. Molecular markers based on retrotransposons have good potential for analysis of genetic diversity given their abundance in the genome. Eight long terminal repeat retrotransposons were selected for the development of inter-retrotransposon-amplified polymorphism markers. To test these primers, we analyzed 32 varieties from Anori, 30 from Manicoré and 10 Mandiocabas from the Manioc Germplasm Bank at Embrapa Western Amazonia. The six informative primer pairs yielded 20- 60 polymorphic bands, averaging 92% polymorphism (51.7-98.4) and 0.37 heterozygosity (0.17 to 0.40), with a Shannon information index of 0.54 (0.26-0.59). These markers can be used to explore the genetic diversity of manioc. © FUNPEC-RP. |
| Databáze: | OpenAIRE |
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