Up-regulation of syncytin-1 contributes to TNF-α-enhanced fusion between OSCC and HUVECs partly via Wnt/β-catenin-dependent pathway
| Autor: | Kai Song, Zhi Xu, Meng Wang, Yong Song, Zhe Shao, Ting-Lin Yan, Ke Liu, Erhui Jiang, Zheng-Jun Shang, Xiaocheng Zhou, Xiao-Ping Zhao, Chun-Ming Huang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Amino Acid Transport System ASC
0301 basic medicine Beta-catenin Pregnancy Proteins Article Cell Line Cell Fusion Minor Histocompatibility Antigens 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Humans Wnt Signaling Pathway beta Catenin Tumor microenvironment Multidisciplinary Cell fusion biology Tumor Necrosis Factor-alpha Chemistry Wnt signaling pathway Endothelial Cells Gene Products env Epithelial Cells Up-Regulation Cell biology 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Catenin biology.protein Tumor necrosis factor alpha |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep40983 |
| Popis: | Accumulating evidence implies that cell fusion is one of the driving forces of cancer invasion and metastasis. However, considerably less is still known about the triggering factors and underlying mechanisms associated with cancer-host cell fusion, particularly in inflammatory tumor microenvironment. In this study, we confirmed that inflammatory factor TNF-α could enhance fusion between squamous cell carcinoma cells 9 (SCC-9) and human umbilical vein endothelial cells (HUVEC). Further study revealed that TNF-α could promote up-regulation of syncytin-1 in SCC-9 and its receptor neutral amino acid transporter type 2 (ASCT-2) in HUVEC. Syncytin-1 acted as an important downstream effector in TNF-α-enhanced cancer-endothelial cell fusion. TNF-α treatment also led to the activation of Wnt/β-catenin signal pathway in SCC-9. The activation of Wnt/β-catenin signal pathway was closely associated with the up-regulation of syncytin-1 in SCC-9 and increased fusion between SCC-9 and HUVEC while blocking of Wnt/β-catenin signal pathway resulted in the corresponding down-regulation of syncytin-1 accompanied by sharp decrease of cancer-endothelial cell fusion. Taking together, our results suggest that Wnt/β-catenin signal pathway activation-dependent up-regulation of syncytin-1 contributes to the pro-inflammatory factor TNF-α-enhanced fusion between oral squamous cell carcinoma cells and endothelial cells. |
| Databáze: | OpenAIRE |
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