An investigation of activities and paraoxon sensitivities of hepatic aliesterases in beta-naphthoflavone-treated rats
| Autor: | Janice E. Chambers, Angela M. Watson, Howard W. Chambers |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Butyrate
Toxicology Valerate Phenylbutyrate Paraoxon Carboxylesterase Substrate Specificity Rats Sprague-Dawley chemistry.chemical_compound beta-Naphthoflavone medicine Animals chemistry.chemical_classification Benzoflavones Chemistry Organophosphate General Medicine Acetylcholinesterase Rats Parathion Biochemistry Liver Female Carboxylic Ester Hydrolases medicine.drug |
| Zdroj: | Toxicology letters. 71(3) |
| ISSN: | 0378-4274 |
| Popis: | Aliesterases (carboxylesterases, EC 3.1.1.1) are serine esterases which may protect acetylcholinesterase during organophosphorus insecticide intoxication by providing alternative phosphorylation sites. Levels of hepatic aliesterase activity were investigated after the intraperitoneal administration of β-naphthoflavone (BNF) to female rats using nine 4-nitrophenyl esters as substrates (including straight and branched chain aliphatic and aromatic esters) and 1-naphthyl acetate. In addition, the in vitro sensitivities of aliesterases to inhibition by paraoxon, the active metabolite of the common insecticide parathion, were studied. Hepatic aliesterases from BNF-treated rats displayed lower activities than those from the controls with all substrates except 4-nitrophenyl phenylbutyrate and isovalerate. The aliesterases from BNF-treated rats were more sensitive to paraoxon inhibition with 4-nitrophenyl phenylbutyrate, valerate, and butyrate. Esterases hydrolyzing 4-nitrophenyl butyrate, valerate, and branched chain esters were most sensitive to paraoxon inhibition while those hydrolyzing 4-nitrophenyl hexanoate and aromatic esters were least sensitive. The results suggested that BNF-induced changes in hepatic aliesterases could alter responses to organophosphates. |
| Databáze: | OpenAIRE |
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