Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines

Autor: Jorge Martínez, Maria Marcela Orozco, Ludmila Sol López Arias, Silvina Elizabeth Wilkowsky, Martina Soledad Paoletta, Eliana Carolina Guillemi, Elena Correa, Valérie Pinarello, Belkis Corona, Marisa Diana Farber, Sofía Ana María de la Fourniere, Javier Fernandez
Přispěvatelé: Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria (INTA), Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, University of Buenos Aires, The Conservation Land Trust, Reserva Experimental Horco Molle, Facultad de Ciencias Naturales, Universidad Nacional de Tucumán (UNT), National Center for Animal and Plant Health, Contrôle des maladies animales exotiques et émergentes (UMR CMAEE), Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2016
Předmět:
0301 basic medicine
Anaplasmosis
bovin
Biodiversité et Ecologie
Genotipos
América del Sur
L73 - Maladies des animaux
phylogeny
0302 clinical medicine
Genotype
Giant anteater
Artiodactyla
2. Zero hunger
0303 health sciences
education.field_of_study
Anaplasma marginale
anaplasma
Infectious Diseases
Tandem Repeat Sequences
Ganado Bovino
wild species
Female
L20 - Écologie animale
P01 - Conservation de la nature et ressources foncières
Erratum
marqueur moléculaire
Bacterial Outer Membrane Proteins
DNA
Bacterial
hôte
Hippocamelus
Genotypes
030231 tropical medicine
Population
Cattle Diseases
Zoology
Taruca
giant anteater
Biology
Host Specificity
Biodiversity and Ecology
03 medical and health sciences
biology.animal
Genetics
phylogénie
medicine
Animals
education
Genotyping
030304 developmental biology
molecular marker
Anaplasma Marginale
Anteater
Research
Sequence Analysis
DNA
Xenarthra
medicine.disease
biology.organism_classification
Genética
030104 developmental biology
Multilocus sequence typing
Cattle
Parasitology
bactérie intracellulaire
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Multilocus Sequence Typing
Zdroj: Parasites and Vectors
Parasites and Vectors, BioMed Central, 2016, 9 (1), 9 p. ⟨10.1186/s13071-016-1653-8⟩
Parasites & Vectors
Parasites and Vectors 1 (9), 9 p.. (2016)
Parasites & Vectors 169 : 305 (2016)
INTA Digital (INTA)
Instituto Nacional de Tecnología Agropecuaria
instacron:INTA
ISSN: 1756-3305
Popis: Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species. Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis. Results: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/ anteater isolate and the bovine group. Inst. de Biotecnología Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: De La Fourniere, Sofia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Orozco, Maria Marcela. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiologia; Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina; Fil: Peña Martínez, Jorge. Conservation Land Trust; Argentina Fil: Correa, Elena. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales. Reserva Experimental Horco Molle; Argentina Fil: Fernández, Javier. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales. Reserva Experimental Horco Molle; Argentina Fil: Lopez Arias, Ludmila Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Corona, Belkis. Centro Nacional de Sanidad Agropecuaria; Cuba Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Pinarello, Valérie. Institut National de la Recherche Agronomique. Contrôle des Maladies Animales Exotiques et Emergentes Centre; Francia
Databáze: OpenAIRE
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