Determination of Free and Conjugated Indole-3-Acetic Acid, Tryptophan, and Tryptophan Metabolites in Grape Must and Wine
| Autor: | Hans J. Köhler, K. Hoenicke, Arnold Schwab, Thomas J. Simat, Hans Steinhart |
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| Rok vydání: | 2001 |
| Předmět: |
Alkaline hydrolysis (body disposal)
Hydrolysis chemistry.chemical_compound Yeasts Aroma compound Vitis Chromatography High Pressure Liquid Aroma Wine Chromatography Indoleacetic Acids biology fungi Tryptophan Reproducibility of Results food and beverages General Chemistry Hydrogen-Ion Concentration biology.organism_classification Biochemistry chemistry Fermentation General Agricultural and Biological Sciences Indole-3-acetic acid |
| Zdroj: | Journal of Agricultural and Food Chemistry. 49:5494-5501 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf010575v |
| Popis: | Tryptophan (Trp) and its metabolites, especially indole-3-acetic acid (IAA), are considered to be potential precursors of 2-aminoacetophenone (AAP), an aroma compound that causes an "untypical aging off-flavor" (UTA) in Vitis vinifera wines. In this study, RP-HPLC with fluorescence detection was used for the qualitative and quantitative analysis of Trp and Trp metabolites in grape musts and wines to which different viticultural measures had been applied (time of harvest, soil treatment, leaf plucking, vine prune). An alkaline hydrolysis was developed to release bound IAA and Trp. A sensitive and selective determination of different Trp metabolites was achieved after solid phase extraction using a strong anion exchange material. In the examined grape musts, more than 95% of the total IAA was bound either as ester conjugate or as amide conjugate. Free IAA and other Trp metabolites were below the detection limit ( |
| Databáze: | OpenAIRE |
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