GAD and IA-2 autoantibody detection in type 1 diabetic patient saliva
| Autor: | Francesco Dotta, N. Sulli, Blegina Shashaj, Elio Vecci, D Masotti, Claudio Tiberti, Federica Lucantoni, Susanna Morano, Antonella Verrienti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Saliva endocrine system diseases Adolescent type 1 diabetes Immunology Radioimmunoassay medicine.disease_cause Sensitivity and Specificity gad autoantibodies Autoimmunity Young Adult human saliva Immunopathology ia-2 autoantibodies medicine Immunology and Allergy Humans First-degree relatives Child Autoantibodies Autoimmune disease Type 1 diabetes biology business.industry Glutamate Decarboxylase Autoantibody Middle Aged Reference Standards medicine.disease stomatognathic diseases Diabetes Mellitus Type 1 Child Preschool biology.protein Regression Analysis Female Antibody business |
| Popis: | Some attempts have been made in assaying glutamic-acid decarboxylase autoantibodies (GADA) in type 1 diabetic patient (T1DM) saliva. However, these salivary assays did not show sufficient sensitivity and specificity in comparison to serum assays. In this study we evaluated the ability of a fluid-phase 35 S-radioimmunoassay to detect GADA and tyrosine phosphatase 2 autoantibodies (IA-2A) in 70 T1DM, 24 T1DM first degree relatives (FDR) and 76 healthy subject saliva. Paired saliva and serum samples were collected from each subject and analyzed. GADA were detected in 45/70 (64.3%) sera and 43/70 (61.4%) T1DM saliva, respectively. IA-2A were detected in 33/70 (47.1%) sera and 30/70 (42.9%) T1DM saliva, respectively. All FDR serum/saliva samples were autoantibody negative. In conclusion, we here report that GADA and IA-2A are detectable with high sensitivity and specificity in human saliva, a specimen which can be easily collected by non-invasive procedures and may represent a reliable tool for the study of T1DM autoimmunity. |
| Databáze: | OpenAIRE |
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