Toxicity of extracellular proteins from Diplodia seriata and Neofusicoccum parvum involved in grapevine Botryosphaeria dieback

Autor: F. Mazet-Kieffer, C. Tarnus, Sibylle Farine, M. Goddard, Christophe Bertsch, Elodie Stempien, Hélène Laloue, Julie Chong, Florence Fontaine, M. Bénard-Gellon, Philippe Larignon, Marjorie Schmitt, Flora Pensec
Přispěvatelé: Laboratoire Vigne Biotechnologie et Environnement EA 3991, Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Laboratoire d'innovation moléculaire et applications (LIMA), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), IFV Pôle Rhône-Méditerranée, Gwangju Institute of Science and Technology (GIST)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Protoplasma
Protoplasma, Springer Verlag, 2015, 252 (2), pp.679-687. ⟨10.1007/s00709-014-0716-y⟩
ISSN: 0033-183X
1615-6102
DOI: 10.1007/s00709-014-0716-y⟩
Popis: Botryosphaeria dieback, esca and Eutypa dieback are three economic major grapevine trunk diseases that cause severe yield reduction in vineyards worldwide. The frequency of disease symptoms has increased considerably over the past decade, and no efficient treatment is currently available to control these diseases. The different fungi associated with grapevine trunk diseases mainly induce necrotic wood and characteristic foliar symptoms. In this context, fungi virulence factors and host invasion are not well understood. We hypothesise that extracellular proteins produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with Botryosphaeria dieback, are virulence factors responsible for the pathogenicity. In our previous work, we demonstrated that the total extracellular compounds produced by N. parvum induced more necrosis on Chardonnay calli and triggered a different defence gene expression pattern than those produced by D. seriata. Furthermore, this aggressiveness was not clearly correlated with the production of mellein, a characteristic phytotoxin of Botryosphaeriaceae, in our in vitro calli model. To characterise other potential virulence factors and to understand the mechanisms of host invasion by the fungus, we evaluated the profile, quantity and the impact of extracellular proteins produced by these fungi on Vitis vinifera calli necrosis and defence gene expression. Our results reveal that, under the same conditions, N. parvum produces more extracellular proteins and in higher concentrations than D. seriata. With Vitis vinifera cv. Chardonnay cells, we showed that equivalent concentrations of proteins secreted by N. parvum were more aggressive than those of D. seriata in producing necrosis and that they clearly induced more grapevine defence genes.
Databáze: OpenAIRE
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