T cell receptor excision circles (TRECs) analysis during acute intrarectal infection of cynomolgus monkeys with pathogenic chimeric simian human immunodeficiency virus
| Autor: | Maria Rosaria Pavone-Cossut, Fausto Titti, Zahra Fagrouch, Roberta Bona, Massimo Ciccozzi, Silvia Baroncelli, Donatella R.M. Negri, Stefania Catone, Viviana Buffa, Jonathan L. Heeney, Pasqualina Leone, Andrea Cara, Iole Macchia |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Cancer Research
T-Lymphocytes T cell CD3 Molecular Sequence Data Simian Acquired Immunodeficiency Syndrome Recent Thymic Emigrant HIV Infections Biology Sequence Homology Nucleic Acid Virology medicine Animals Humans Gene Base Sequence Chimera T-cell receptor excision circles Simian human immunodeficiency virus T-cell receptor Genes T-Cell Receptor Macaca fascicularis Infectious Diseases medicine.anatomical_structure Immunology HIV-1 biology.protein Simian Immunodeficiency Virus DNA Circular CD8 |
| Zdroj: | Virus Research. 126:86-95 |
| ISSN: | 0168-1702 |
| Popis: | Several studies have shown the importance of evaluating Recent Thymic Emigrants (RTEs) by quantification of T cell receptor-rearrangement excision circles (TRECs), as a measure of de novo T cell generation during human immunodeficiency virus-1 (HIV-1) infection. To determine whether acute viral infection may have an impact on TRECs, cynomolgus monkeys (Macaca fascicularis) were infected intrarectally with simian human immunodeficiency virus (SHIV) 89.6P(cy11) and the number of signal-joint (sj) TRECs was determined in purified CD4+ and CD8+ populations for up to 28 weeks post-infection. Four weeks after infection, TRECs levels significantly decreased in both CD3+ CD4+ and in CD3+ CD8+ T lymphocytes of infected monkeys, whereas they remained unchanged in uninfected animals. This reduction was followed by a progressive TRECs number recovery in CD3+ CD4+ T lymphocytes that positively correlated with changes in the levels of circulating CD3+ CD4+ T cells. In the CD3+ CD8+ T cell subset, TRECs number remained significantly low and inversely correlated with the increase in the percentages of CD3+ CD8+ T cells. These data suggest that SHIV89.6P(cy11) intrarectal infection of cynomolgus monkeys differently affects TRECs content in CD3+ CD4+ and CD3+ CD8+ T cell subsets. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect