Intestinal anti-inflammatory effects of total alkaloid extract from Fumaria capreolata in the DNBS model of mice colitis and intestinal epithelial CMT93 cells
| Autor: | Fadila Maiza, Alba Rodríguez-Nogales, Teresa Vezza, José Garrido-Mesa, Antonio Segura-Carretero, Julio Gálvez, María del Mar Contreras, María Elena Rodríguez-Cabezas, Francesca Algieri, Noureddine Bribi, M. P. Utrilla |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Coptisine medicine.drug_class Anti-Inflammatory Agents Pharmaceutical Science Pharmacology Anti-inflammatory Cell Line 03 medical and health sciences chemistry.chemical_compound Mice Alkaloids In vivo Drug Discovery medicine Animals Immunologic Factors Colitis biology business.industry Interleukin-6 Plant Extracts Tumor Necrosis Factor-alpha Fumaria Epithelial Cells biology.organism_classification medicine.disease In vitro 030104 developmental biology Complementary and alternative medicine chemistry Biochemistry Fumaria capreolata Molecular Medicine Protopine Cytokines Dinitrofluorobenzene business |
| Zdroj: | Phytomedicine : international journal of phytotherapy and phytopharmacology. 23(9) |
| ISSN: | 1618-095X |
| Popis: | Background Fumaria capreolata L. (Papaveraceae) is a botanical drug used in North Africa for its gastro-intestinal and anti-inflammatory properties. It is characterized for the presence of several alkaloids that could be responsible for some of its effects, including an immunomodulatory activity. Purpose To test in vivo the intestinal anti-inflammatory properties of the total alkaloid fraction extracted from the aerial parts of F. capreolata (AFC), and to evaluate its effects on an intestinal epithelial cell line. Study design and methods AFC was chemically characterized by liquid chromatography coupled to diode array detection and high resolution mass spectrometry. Different doses of AFC (25, 50 and 100 mg/kg) were assayed in the DNBS model of experimental colitis in mice, and the colonic damage was evaluated both histologically and biochemically. In addition, in vitro experiments were performed with this alkaloid fraction on the mouse intestinal epithelial cell line CMT93 stimulated with LPS. Results The chemical analysis of AFC revealed the presence of 23 alkaloids, being the most abundants stylopine, protopine and coptisine. Oral administration of AFC produced a significant inhibition of the release and the expression of IL-6 and TNF- α in the colonic tissue. It also suppressed in vivo the transcription of other pro-inflammatory mediators such as IL-1 β , iNOS, IL-12 and IL-17. Furthermore, AFC showed an immunomodulatory effect in vitro since it was able to inhibit the mRNA expression of IL-6, TNF- α and ICAM-1. Moreover, the beneficial effect of AFC in the colitic mice could also be associated with the normalization of the expression of MUC-2 and ZO-1, which are important for the intestinal epithelial integrity. Conclusion The present study suggests that AFC, containing 1.3% of stylopine and 0.9% of protopine, significantly exerted intestinal anti-inflammatory effects in an experimental model of mouse colitis. This fact could be related to a modulation of the intestinal immune response and a restoration of the intestinal epithelial function. |
| Databáze: | OpenAIRE |
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