Re-formulation of selected DNA vaccine candidates and their evaluation as protein vaccines using a guinea pig aerosol infection model of tuberculosis
| Autor: | Julia Vipond, Richard Vipond, Julia A. Tree, Philip Marsh, Else Marie Agger, Ann Williams, Simon O. Clark, Graham J. Hatch |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Tuberculosis medicine.medical_treatment Genetic Vectors Guinea Pigs Immunology Lymphocyte proliferation Microbiology DNA vaccination Guinea pig Mycobacterium tuberculosis Epitopes Immune system Adjuvants Immunologic Escherichia coli Vaccines DNA medicine Animals Tuberculosis Vaccines Lung Tuberculosis Pulmonary Cells Cultured Cell Proliferation Antigens Bacterial Immunity Cellular biology Th1 Cells biology.organism_classification medicine.disease Antibodies Bacterial Virology Vaccination Disease Models Animal Infectious Diseases Genes Bacterial Immunoglobulin G Vaccines Subunit Female Adjuvant |
| Zdroj: | Tuberculosis. 86:218-224 |
| ISSN: | 1472-9792 |
| Popis: | Summary A selection of previously identified protective Mycobacterium tuberculosis DNA vaccines were re-formulated as proteins and administered with a Th1-inducing adjuvant to help stimulate the relevant immune responses necessary for protection. All three candidate-vaccines conferred high levels of antigen-specific cellular and humoral responses, as indicated by lymphocyte proliferation and serum IgG levels. Protective efficacy was also assessed in comparison with the current vaccine, BCG (the ‘gold-standard' against which new vaccines are tested), and a saline (negative) control. One candidate (Rv1806-1807) induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M. tuberculosis in the lungs. |
| Databáze: | OpenAIRE |
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