Metabolomic study reveals key metabolic adjustments in the xerohalophyte Salvadora persica L. during adaptation to water deficit and subsequent recovery conditions
| Autor: | Asish Kumar Parida, Jaykumar Rangani, Ashok Panda |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Drought tolerance Phenylalanine Plant Science 01 natural sciences Desiccation tolerance 03 medical and health sciences chemistry.chemical_compound Stress Physiological Tandem Mass Spectrometry Salvadora persica Genetics Metabolomics Gallic acid chemistry.chemical_classification biology Salvadoraceae fungi Water food and beverages biology.organism_classification Adaptation Physiological Droughts Amino acid 030104 developmental biology chemistry Biochemistry Osmoprotectant Malic acid 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry. 150:180-195 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2020.02.036 |
| Popis: | Water deficit severely limits productivity of plants, and pose a major threat to modern agriculture system. Therefore, understanding drought adaptive mechanisms in drought-tolerant plants is imperative to formulate strategies for development of desiccation tolerance in crop plants. In present investigation, metabolic profiling employing GC-QTOF-MS/MS and HPLC-DAD was carried out to evaluate metabolic adjustments under drought stress in the xero-halophyte Salvadora persica. The metabolite profiling identified a total of 68 metabolites in S. persica leaf, including organic acids, amino acids, sugars, sugar alcohols, hormones, and polyphenols. The results showed that higher cellular osmolality under drought stress was accompanied by accumulations of several osmoprotectants like sugars and polyols (sucrose, glucose, mannose, galactose, erythrose, sorbose, glycerol, and myoinositol), organic acids (galactaric acid, tartaric acid, malic acid, oxalic acid, and citric acid), and amino acids (alanine, phenylalanine, tyrosine). Upregulation of ABA and JA support to achieve early drought tolerance in S. persica. Moreover, accumulation of coumarin, gallic acid, and chlorogenic acid provide antioxidative defense to S. persica. KEGG pathway enrichment analysis showed that altered metabolites were associated with starch and sucrose metabolism, galactose metabolism, inositol phosphate metabolism, and phenylalanine metabolism. While during recovery, metabolites associated with lysine biosynthesis and alanine, aspartate and glutamate metabolism were significantly altered. The results of the present study imply that coordinated regulations between various metabolites, metabolic processes, and pathways empower the xerohalophyte S. persica to adapt under drought environment. The knowledge from this study will enable the development of drought tolerance in crops using genetic engineering and breeding approaches. |
| Databáze: | OpenAIRE |
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