Genetics and biochemistry remain essential in the structural era of the spliceosome
| Autor: | Megan Mayerle, Christine Guthrie |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics Spliceosome RNA Splicing Clinical Sciences Eukaryota High resolution RNA MOLECULAR BIOLOGY METHODS Biology Biochemistry Introns Article General Biochemistry Genetics and Molecular Biology Molecular Imaging 03 medical and health sciences 030104 developmental biology RNA splicing RNA Precursors Spliceosomes Humans Generic health relevance Molecular Biology |
| Zdroj: | Methods. 125:3-9 |
| ISSN: | 1046-2023 |
| DOI: | 10.1016/j.ymeth.2017.01.006 |
| Popis: | The spliceosome is not a single macromolecular machine. Rather it is a collection of dynamic heterogeneous subcomplexes that rapidly interconvert throughout the course of a typical splicing cycle. Because of this, for many years the only high resolution structures of the spliceosome available were of smaller, isolated protein or RNA components. Consequently much of our current understanding of the spliceosome derives from biochemical and genetic techniques. Now with the publication of multiple, high resolution structures of the spliceosome, some question the relevance of traditional biochemical and genetic techniques to the splicing field. We argue such techniques are not only relevant, but vital for an in depth mechanistic understanding of pre-mRNA splicing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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